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产品描述：人解整合素样金属蛋白酶12(ADAM12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液、组织匀浆、细胞裂解物样本中的ADAM12含量。ADAM12是一种解整合素和金属蛋白酶，属于ADAM家族，在细胞黏附、信号传导等过程中发挥重要作用。研究发现，ADAM12在多种癌症中表达异常，尤其在胶质瘤中高表达，与预后密切相关，可能成为预测胶质瘤恶性程度和预后的生物标志物，并为胶质瘤治疗提供新的潜在靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于体外研究ADAM12蛋白的表达调控机制，探索其在癌症生物学、纤维化疾病模型或生殖系统研究中的功能，为细胞实验、动物模型及临床前样本分析提供定量检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAM12; MLTN; UNQ346/PRO545; Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12; Meltrin-alpha
缩写：ADAM12
Uniprot No.：O43184
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:20 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:40 | Average % | 102 | ||||
| Range % | 95-108 | |||||
回收率：
| The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-93 | ||||
| EDTA plasma (n=4) | 91 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8712.8022.8372.65510002.3862.3162.3512.1695001.6631.7611.7121.5302501.0581.0231.0410.8591250.6750.6240.6500.46862.50.4130.4090.4110.22931.250.2190.2250.2220.04000.1840.1790.182 | ||||||
货期：3-5 working days
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