首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人腺苷脱氨酶(ADA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的ADA含量。ADA即腺苷脱氨酶，它参与嘌呤代谢，在维持细胞内腺苷水平平衡等方面起重要作用。研究机制上，其表达与活性异常和多种疾病相关，如免疫缺陷病等，目前通过对其活性调节、结构功能研究等，为相关疾病的治疗提供思路。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，适用于免疫调节机制研究、肝脏疾病模型构建、感染性疾病中ADA动态变化监测等科研场景，为探究ADA在代谢调控、免疫应答及病理进程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ada ELISA Kit; ADA_HUMAN ELISA Kit; ADA1 ELISA Kit; Adenosine aminohydrolase ELISA Kit; Adenosine deaminase ELISA Kit
缩写：ADA
Uniprot No.：P00813
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of human ADA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected1002.6942.7522.7232.602502.0822.1922.1372.016251.4181.3461.3821.26112.50.8910.9170.9040.7836.250.5400.5320.5360.4153.120.3500.3710.3610.2401.560.2760.2990.2880.16700.1240.1170.121 | ||||||
货期：3-5 working days
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