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产品描述：人解整合素样金属蛋白酶10(ADAM10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAM10含量。ADAM10 属去整合素金属蛋白酶家族，是重要的膜蛋白水解酶。其可裂解多种底物参与细胞信号传导、细胞黏附等。研究发现它与肿瘤、神经退行性疾病等相关，是潜在治疗靶点，目前针对其作用机制及药物研发正不断深入。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于肿瘤发生机制、炎症反应调控及神经退行性疾病等基础研究领域，特别适合体外实验中对细胞微环境中ADAM10的动态监测或生物标志物筛查本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAM10; KUZ; MADM; Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD antigen CD156c
缩写：ADAM10
Uniprot No.：O14672
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 98-109 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of human ADAM10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.0531.9812.0171.9182501.5681.5451.5571.4581250.8230.8110.8170.71862.50.4200.4440.4320.33331.20.2730.2850.2790.18015.60.1780.1820.1800.0817.80.1270.1310.1290.03000.0980.1000.099 | ||||||
货期：3-5 working days
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