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产品描述：人肿瘤坏死因子α转化酶(TACE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ADAM17含量。ADAM17是一种重要的靶点。它属于解聚素和金属蛋白酶家族，在细胞信号传导、炎症反应等过程中发挥关键作用。研究主要聚焦于其蛋白水解活性机制，通过切割膜蛋白来调控细胞功能，在癌症、炎症性疾病等治疗研究中有重要意义。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本产品可用于研究免疫调节、肿瘤微环境及代谢性疾病，满足基础科研中对疾病模型、药物干预实验或信号通路研究的定量分析需求，为探索TACE在炎症性疾病、肿瘤发生发展中的调控机制提供可靠工具，适用于体外实验中的蛋白表达水平评估及相关分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAM17; CSVP; TACE; Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD antigen CD156b
缩写：ADAM17
Uniprot No.：P78536
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human TACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40001.9311.8721.9021.770 20001.6481.5961.6221.490 10001.3351.3041.3201.188 5001.0821.0121.0470.915 2500.6430.6090.6260.494 1250.4090.3790.3940.262 62.50.2740.2570.2660.134 00.1350.1280.132 | ||||||
货期：3-5 working days
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