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产品描述：猪促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTH含量。促肾上腺皮质激素（ACTH）由腺垂体分泌，主要作用是促进肾上腺皮质增生、合成与分泌糖皮质激素。在研究机制上，它通过与肾上腺皮质细胞膜上受体结合，激活腺苷酸环化酶-cAMP-蛋白激酶系统发挥作用，对其研究有助于深入了解内分泌调控机制及相关疾病。试剂盒检测范围为6.25 pg/mL-100 pg/mL，该产品适用于动物生理机制研究、疾病模型内分泌功能评估或药物干预实验中的激素水平监测，例如探究应激条件下ACTH动态变化、肾上腺相关病理机制或药效学评价等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-100 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-87 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of pig ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-99 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 1000.1770.1880.183 500.3320.3440.338 250.6310.6340.633 12.51.2781.2391.259 6.252.3562.4782.417 | ||||||
货期：3-5 working days
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