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产品描述：人乙酰胆碱酯酶(AChE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACHE含量。ACHE即乙酰胆碱酯酶，是重要的神经递质相关靶点。它主要负责水解乙酰胆碱以终止神经冲动传递。在研究中，针对ACHE的作用机制，常开发抑制药物，用于治疗如阿尔茨海默病等神经系统疾病，调节胆碱能神经功能。试剂盒检测范围为0.94 mU/mL-60 mU/mL，适用于探索神经系统疾病模型、环境毒素暴露评估或药物干预实验中AChE的动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACEE ELISA Kit; ACES_HUMAN ELISA Kit; Acetylcholinesterase ELISA Kit; AChE ELISA Kit; Apoptosis related acetylcholinesterase ELISA Kit; ARACHE ELISA Kit; N ACHE ELISA Kit; N-ACHE ELISA Kit; YT ELISA Kit; YT blood group ELISA Kit
缩写：ACHE
Uniprot No.：P22303
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.94 mU/mL-60 mU/mL
灵敏度：0.23 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 91 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected602.4622.5462.5042.385302.1762.2432.2102.091151.9041.9051.9051.7867.51.4391.4731.4561.3373.750.8550.8240.8400.7211.880.4780.4590.4690.3500.940.2670.2490.2580.13900.1200.1170.119 | ||||||
货期：3-5 working days
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