首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠二胺氧化酶(DAO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DAO含量。DAO即去中心化自治组织，背景上是区块链技术发展的产物，旨在以智能合约实现组织的自治管理。其研究机制聚焦于通过代码自动执行规则，减少人为干预，提升决策效率与透明度，吸引各界探索其在金融、治理等领域的应用可能。试剂盒检测范围为15.6 mU/mL-1000 mU/mL，适用于科研实验中DAO水平的动态监测，例如在肠道损伤模型、代谢综合征研究或药物干预实验中，通过检测不同生理/病理条件下血清、血浆及组织样本的DAO表达量，为探索肠道屏障功能调节机制、炎症相关疾病病理进程提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aoc1 ELISA kit; Abp1Amiloride-sensitive amine oxidase [copper-containing] ELISA kit; DAO ELISA kit; Diamine oxidase ELISA kit; EC 1.4.3.22 ELISA kit; Amiloride-binding protein 1 ELISA kit; Amine oxidase copper domain-containing protein 1 ELISA kit; Histaminase ELISA kit
缩写：ABP1
Uniprot No.：Q8JZQ5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 mU/mL-1000 mU/mL
灵敏度：3.9 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-87 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 109 | 104-112 | ||||
| EDTA plasma (n=4) | 85 | 81-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected10002.8792.7522.8162.6075002.3012.2162.2592.0502501.5841.5601.5721.3631250.9450.9320.9390.73062.50.6550.6720.6640.45531.20.5320.5420.5370.32815.60.4120.4070.4100.20100.2110.2060.209 | ||||||
本试剂盒所含材料：
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A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse DAO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DAO antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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