首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人腺苷三磷酸结合盒转运体G2(ABCG2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织培养上清液样本中的ABCG2含量。ABCG2是重要的药物外排转运蛋白，属ATP结合盒（ABC）转运蛋白超家族。它在多种组织有表达，和肿瘤多药耐药相关。其研究机制主要聚焦于底物识别机制、调控机制等，旨在通过抑制它克服肿瘤耐药，为癌症治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究肿瘤耐药机制、药物代谢动力学及干细胞功能调控等科研领域，支持科研人员在药物开发、肿瘤生物学或分子药理学研究中高效评估ABCG2的表达动态，为探索跨膜转运蛋白功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ABCG2; ABCP; BCRP; BCRP1; MXR; Broad substrate specificity ATP-binding cassette transporter ABCG2; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD antigen CD338
缩写：ABCG2
Uniprot No.：Q9UNQ0
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ABCG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 82-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of human ABCG2 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 104 | 96-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8562.7992.8282.71451.9442.1112.0281.9142.51.2221.2551.2391.1251.250.7740.7510.7630.6490.6250.4580.4620.4600.3460.3120.3040.3030.3040.1900.1560.2250.2270.2260.11200.1130.1150.114 | ||||||
货期：3-5 working days
×
