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产品描述：人血管紧张素转化酶2(ACE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的ACE2含量。ACE2（血管紧张素转换酶2）是一种位于人体细胞表面的膜蛋白，作为SARS-CoV-2病毒的主要受体，其与病毒表面的S蛋白结合是病毒进入细胞的关键。研究显示，ACE2在SARS-CoV-2感染中起重要作用，其动态表达变化可能与COVID-19的发病机制相关，且ACE2及其信号通路上的关键分子在肺部炎症发生及修复中发挥重要作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于体外实验研究，可应用于心血管疾病模型、病毒感染机制探索、药物靶点验证等科研场景，例如通过检测细胞培养上清中ACE2的动态变化研究病毒入侵机制，或分析不同病理条件下生物液体样本中ACE2的表达水平差异。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACE 2 ELISA Kit; ACE related carboxypeptidase ELISA Kit; ACE-related carboxypeptidase ELISA Kit; ACE2 ELISA Kit; ACE2_HUMAN ELISA Kit; ACEH ELISA Kit; Angiotensin converting enzyme 2 ELISA Kit; Angiotensin converting enzyme homolog ELISA Kit; Angiotensin converting enzyme like protein ELISA Kit; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 ELISA Kit; Angiotensin I converting enzyme 2 ELISA Kit; Angiotensin-converting enzyme homolog ELISA Kit; DKFZP434A014 ELISA Kit; EC 3.4.17 ELISA Kit; metalloprotease MPROT 15 ELISA Kit; Metalloprotease MPROT15 ELISA Kit; OTTHUMP00000022963 ELISA Kit; Processed angiotensin-converting enzyme 2 ELISA Kit
缩写：ACE2
Uniprot No.：Q9BYF1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-107 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2162.1822.1991.95552.0121.9161.9641.7202.51.7541.6431.6991.4551.251.3261.2901.3081.0640.6251.0080.9440.9760.7320.3120.7140.6760.6950.4510.1560.4160.3920.4040.16000.2490.2390.244 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human AE2 antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ACE2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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