首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人乙酰CoA羧化酶1(ACACA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ACACA含量。ACACA（乙酰辅酶A羧化酶α）是一种多功能酶，催化乙酰辅酶A的羧基化，生成丙二酰辅酶A，是脂肪酸合成的限速步骤。其研究机制涉及生物素酶的催化作用和脂肪酸代谢调控，与心血管、代谢和癌症等领域相关。试剂盒检测范围为9.38 pg/mL-600 pg/mL，适用于科研场景中探索ACACA在能量代谢调控机制、代谢相关疾病模型中的表达变化，或用于药物筛选过程中靶点效应的定量评估。为脂质代谢基础研究及转化医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACAC ELISA Kit; ACACA ELISA Kit; ACACA_HUMAN ELISA Kit; ACACB ELISA Kit; ACC alpha ELISA Kit; ACC ELISA Kit; ACC beta ELISA Kit; ACC-alpha ELISA Kit; ACC1 ELISA Kit; ACC2 ELISA Kit; ACCA ELISA Kit; ACCB ELISA Kit; Acetyl CoA carboxylase 1 ELISA Kit; Acetyl CoA carboxylase 2 ELISA Kit; Acetyl CoA carboxylase alpha ELISA Kit; Acetyl CoA carboxylase beta ELISA Kit; Acetyl Coenzyme A carboxylase alpha ELISA Kit; Acetyl Coenzyme A carboxylase beta ELISA Kit; Biotin carboxylase ELISA Kit; COA1 ELISA Kit; COA2 ELISA Kit; HACC275 ELISA Kit; OTTHUMP00000164069 ELISA Kit; OTTHUMP00000164070 ELISA Kit; OTTHUMP00000164076 ELISA Kit; OTTHUMP00000240532 ELISA Kit
缩写：ACACA
Uniprot No.：Q13085
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：9.38 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACACA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 98 | |||||
| Range % | 96-102 | ||||||
| 1:10 | Average % | 107 | |||||
| Range % | 103-114 | ||||||
| 1:20 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:40 | Average % | 88 | |||||
| Range % | 86-95 | ||||||
回收率：
| The recovery of human ACACA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 99-108 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 6002.1692.2022.1862.004 3001.7021.8141.7581.576 1501.2021.2541.2281.046 750.7110.7470.7290.547 37.50.5030.5060.5050.323 18.750.2840.2980.2910.109 9.380.2360.2490.2430.061 00.1740.1890.182 . | |||||||
货期：3-5 working days
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