首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管紧张素Ⅰ转化酶(ACEⅠ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ACE含量。ACE即血管紧张素转换酶，在肾素-血管紧张素-醛固酮系统中发挥关键作用。它能将血管紧张素Ⅰ转化为血管紧张素Ⅱ，使血管收缩、血压升高。目前关于ACE的研究多围绕其抑制剂展开，以探索控制血压、治疗心血管疾病的新途径。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，支持科研人员探究ACEⅠ在高血压、肺纤维化、代谢综合征等疾病中的病理机制，或用于评估药物干预对ACEⅠ活性及表达水平的影响；适用于基础医学研究、疾病模型分析以及生物制剂开发等科研场景，为心血管及呼吸系统相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACE 1 ELISA Kit; ACE ELISA Kit; ACE T ELISA Kit; ACE_HUMAN ELISA Kit; ACE1 ELISA Kit; Angiotensin converting enzyme somatic isoform ELISA Kit; Angiotensin converting enzyme testis specific isoform ELISA Kit; Angiotensin I converting enzyme 1 ELISA Kit; Angiotensin I converting enzyme ELISA Kit; Angiotensin I converting enzyme peptidyl dipeptidase A 1 ELISA Kit; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript ELISA Kit; Angiotensin-converting enzyme ELISA Kit; Carboxycathepsin ELISA Kit; CD 143 ELISA Kit; CD143 ELISA Kit; CD143 antigen ELISA Kit; DCP 1 ELISA Kit; DCP ELISA Kit; DCP1 ELISA Kit; Dipeptidyl carboxypeptidase 1 ELISA Kit; Dipeptidyl carboxypeptidase I ELISA Kit; Kininase II ELISA Kit; MGC26566 ELISA Kit; MVCD3 ELISA Kit; Peptidase P ELISA Kit; Peptidyl dipeptidase A ELISA Kit; soluble form ELISA Kit; Testicular ECA ELISA Kit
缩写：ACE
Uniprot No.：P12821
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 82-98 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 99-111 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-98 | ||||
| EDTA plasma (n=4) | 112 | 105-117 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.8442.7522.7982.6636002.5362.3822.4592.3243001.9161.9851.9511.8161501.3901.3551.3731.238750.7150.7640.7400.60537.50.3860.3750.3810.24618.750.2220.2390.2310.09600.1320.1370.135 | ||||||
货期：3-5 working days
×
