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产品描述：人酪氨酸蛋白激酶ABL1(ABL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ABL1含量。ABL1是一种原癌基因，位于9号染色体，编码非受体酪氨酸激酶。其突变或过度表达可导致细胞增殖失控，与多种癌症相关。研究显示，ABL1通过激活信号通路促进肿瘤细胞生长和扩散。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于科研领域中对ABL1蛋白表达水平的定量分析，如白血病相关分子机制研究、酪氨酸激酶抑制剂药物筛选、肿瘤细胞信号通路调控分析等基础研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Abelson murine leukemia viral oncogene homolog 1 ELISA Kit; Abelson tyrosine protein kinase 1 ELISA Kit; Abl 1 ELISA Kit; ABL ELISA Kit; ABL proto oncogene 1 non receptor tyrosine kinase ELISA Kit; ABL1 ELISA Kit; ABL1_HUMAN ELISA Kit; bcr/abl ELISA Kit; bcr/c abl oncogene protein ELISA Kit; c ABL ELISA Kit; c abl oncogene 1 non receptor tyrosine kinase ELISA Kit; c abl oncogene 1 receptor tyrosine kinase ELISA Kit; c ABL1 ELISA Kit; JTK7 ELISA Kit; p150 ELISA Kit; Proto oncogene tyrosine protein kinase ABL1 ELISA Kit; Proto-oncogene c-Abl ELISA Kit; Tyrosine-protein kinase ABL1 ELISA Kit; v abl Abelson murine leukemia viral oncogene homolog 1 ELISA Kit; v abl ELISA Kit
缩写：ABL1
Uniprot No.：P00519
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ABL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 85-102 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 95-114 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 80-107 | ||||||
| 1:8 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
回收率：
| The recovery of human ABL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-97 | |||||
| EDTA plasma (n=4) | 87 | 80-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.2872.3122.3002.1108001.5631.6561.6101.4204001.0251.0541.0400.8502000.6440.6550.6500.4601000.4480.4740.4610.271500.3110.3250.3180.128250.2890.2880.2890.09900.1890.1900.190 | |||||||
货期：3-5 working days
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