Mouse Angiotensin converting enzyme 2(ACE2)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E17204m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血管紧张素转化酶2(ACE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ACE2含量。ACE2是血管紧张素转换酶2，在多种组织中表达。其能参与肾素 - 血管紧张素系统的调节，维持血管稳态。在医学研究里，它还是新冠病毒等病原体的重要入侵靶点，以其为靶点的研究对防治相关疾病意义重大。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于心血管疾病模型、病毒感染机制、药物筛选等基础研究领域，尤其可用于评估基因编辑动物模型中ACE2表达水平变化或体外药物干预对酶活性的影响，为病理机制探索和化合物疗效验证提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Ace2 ELISA Kit; Angiotensin-converting enzyme 2 ELISA Kit; EC 3.4.17.23 ELISA Kit; ACE-related carboxypeptidase) [Cleaved into: Processed angiotensin-converting enzyme 2] ELISA Kit
缩写：
ACE2
Uniprot No.：
Q8R0I0
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, cell culture supernates, tissue homogenates
检测范围：
6.25 pg/mL-400 pg/mL
灵敏度：
1.56 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-99
1:2	Average %	89
	Range %	87-93
1:4	Average %	90
	Range %	85-96
1:8	Average %	96
	Range %	92-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
400	2.5266	2.5021	2.514	2.356
200	1.7845	1.7966	1.791	1.633
100	1.2961	1.2124	1.254	1.096
50	0.8509	0.7926	0.822	0.664
25	0.5774	0.5513	0.564	0.406
12.5	0.3821	0.3542	0.368	0.210
6.25	0.2759	0.2407	0.258	0.100
0	0.1631	0.1538	0.158