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产品描述：人ATP-结合盒式亚家族C成员5 (ABCC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ABCC5含量。ABCC5是重要靶点。它属于ATP结合盒转运蛋白家族，在细胞内物质转运等方面有作用。研究机制上，聚焦于其底物特异性、对药物代谢及细胞信号的影响等，以明确其在疾病发生发展过程的角色，为相关疾病治疗提供新方向。试剂盒检测范围为0.312 pmol/ml-20 pmol/ml，本产品适用于基础医学研究中ABCC5蛋白表达水平的定量分析，包括肿瘤耐药机制探索、药物代谢动力学研究及基因功能验证等方向本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ABC 33 ELISA Kit; ABC33 ELISA Kit; ABCC 5 ELISA Kit; Abcc5 ELISA Kit; ATP binding cassette sub family C (CFTR/MRP) member 5 ELISA Kit; ATP binding cassette sub family C member 5 ELISA Kit; ATP-binding cassette sub-family C member 5 ELISA Kit; Canalicular multispecific organic anion transporter C ELISA Kit; DKFZp686C1782 ELISA Kit; EST277145 ELISA Kit; MOAT C ELISA Kit; MOAT-C ELISA Kit; MOATC ELISA Kit; MRP 5 ELISA Kit; MRP5_HUMAN ELISA Kit; Multi specific organic anion tranporter C ELISA Kit; Multi-specific organic anion transporter C ELISA Kit; Multidrug resistance associated protein 5 ELISA Kit; Multidrug resistance-associated protein 5 ELISA Kit; pABC 11 ELISA Kit; pABC11 ELISA Kit; SMRP ELISA Kit
缩写：ABCC5
Uniprot No.：O15440
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 pmol/ml-20 pmol/ml
灵敏度：0.078 pmol/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SMRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 92-112 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of human SMRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-105 | ||||
| EDTA plasma (n=4) | 89 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected 202.7952.6032.6992.549 102.3202.1922.2562.106 51.5641.5451.5551.405 2.50.9130.8780.8960.746 1.250.5150.5010.5080.358 0.6250.2890.2790.2840.134 0.3120.2270.2210.2240.074 00.1510.1490.150 | ||||||
货期：3-5 working days
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