首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人芳烷基胺N-乙酰转移酶(AANAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AANAT含量。AANAT（褪黑素合成酶）是褪黑素合成过程中的关键酶，受光周期调控，参与调节昼夜节律和多种生理过程。研究机制涉及褪黑素合成、昼夜节律调节及睡眠等生理功能的调控。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可将其应用于神经生物学、时间生物学或内分泌学领域的机制研究，例如探究不同光照周期下 AANAT 的节律性表达、评估褪黑激素通路相关疾病的动物模型或细胞模型中酶活性变化，以及筛选影响生物钟调控的小分子化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA-NAT ELISA Kit; Aanat ELISA Kit; Aralkylamine N-acetyltransferase ELISA Kit; Arylalkylamine N acetyltransferase ELISA Kit; Serotonin acetylase ELISA Kit; Serotonin N-acetyltransferase ELISA Kit; SNAT ELISA Kit; SNAT_HUMAN ELISA Kit
缩写：AANAT
Uniprot No.：Q16613
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AANAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 103-109 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-98 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 86-96 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human AANAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-108 | |||||
| EDTA plasma (n=4) | 92 | 87-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.1412.3022.2222.12110001.6261.7111.6691.5685001.2481.3581.3031.2022500.7910.7600.7760.6751250.5310.5030.5170.41662.50.3110.3310.3210.22031.250.1900.1980.1940.09300.0970.1050.101 | |||||||
货期：3-5 working days
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