Rat α2 macroglobulin,α2 MG ELISA Kit

货号: IPD-E13863r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：大鼠α2巨球蛋白(α2-MG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的A2M含量。A2M（α-2-巨球蛋白）是一种在血浆中含量丰富的糖蛋白，具有蛋白酶抑制作用，参与急性炎症反应。其研究机制涉及与多种免疫炎症因子结合，调节体内小分子平衡。在神经系统疾病中，A2M与阿尔茨海默病、多发性硬化等疾病相关，可能通过空间捕获蛋白酶来抑制其活性。试剂盒检测范围为3.13 ng/mL-800 ng/mL，该产品专为科研领域设计，可应用于炎症机制研究、肝肾功能评估、疾病动物模型分析及药物干预效果评价等方向，为探索α2 - MG在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
缩写：A2M
Uniprot No.：P06238
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：3.13 ng/mL-800 ng/mL
灵敏度：3.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	88-105
1:2000	Average %	91
	Range %	89-102
1:4000	Average %	93
	Range %	90-98
1:8000	Average %	94
	Range %	87-98

回收率：

The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2Average8000.2180.2140.2162000.4510.4610.456500.8500.8610.85612.51.2251.2421.2343.1251.7261.7371.73201.9441.9671.956

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