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产品描述：人P糖蛋白/渗透性糖蛋白(P-gp/ABCB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、腹水、组织匀浆样本中的ABCB1含量。ABCB1（也称为MDR1或P糖蛋白）是一种跨膜蛋白，主要功能是利用ATP水解产生的能量将底物主动转出细胞膜。其在多药耐药中起关键作用，通过将化疗药物泵出细胞外，降低细胞内药物浓度，导致肿瘤细胞对多种化疗药物产生耐药性。研究ABCB1的机制有助于开发新的治疗方法，克服多药耐药性。试剂盒检测范围为3.12 ng/mL-200 ng/mL，本试剂盒可为肿瘤生物学、药理学及分子毒理学研究提供可靠工具，支持科研人员探索P-gp在疾病模型中的表达动态、药物相互作用机制以及新型抑制剂开发等研究方向，满足基础研究与药物开发中的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ABC20 ELISA Kit; ABCB1 ELISA Kit; ATP binding cassette, sub family B (MDR/TAP), member 1 ELISA Kit; ATP-binding cassette sub-family B member 1 ELISA Kit; CD243 ELISA Kit; CLCS ELISA Kit; Colchicin sensitivity ELISA Kit; Doxorubicin resistance ELISA Kit; GP170 ELISA Kit; MDR1 ELISA Kit; MDR1_HUMAN ELISA Kit; Multidrug resistance 1 ELISA Kit; Multidrug resistance protein 1 ELISA Kit; P glycoprotein 1 ELISA Kit; P gp ELISA Kit; P-glycoprotein 1 ELISA Kit; PGY1 ELISA Kit
缩写：ABCB1
Uniprot No.：P08183
种属：Homo sapiens (Human)
样本类型：serum, plasma, ascitic fluid, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ABCB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 86-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human ABCB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-106 | ||||
| EDTA plasma (n=4) | 95 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.7672.8582.8132.707 1002.4622.4762.4692.363 501.9421.9341.9381.832 251.2231.2451.2341.128 12.50.6040.6320.6180.512 6.250.3410.3390.3400.234 3.120.2480.2590.2540.148 00.1070.1050.106 | ||||||
货期：3-5 working days
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