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产品描述：人6酮前列腺素F1a(6-keto-PGF1a)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的6ketoPGF1a含量。人6酮前列腺素F1a（6-keto-PGF1a）是一种由前列腺素I2（PGI2）代谢生成的稳定产物，其在人体内具有调节血管功能和血小板聚集的作用。6-keto-PGF1a的研究背景主要涉及其在心血管疾病、炎症反应和肿瘤发生发展中的作用机制。试剂盒检测范围为50 pg/mL-800 pg/mL，适用于心血管疾病机制研究、药物开发中前列环素代谢评估以及内皮功能相关实验，可为血管生物学、药理学及病理生理学研究提供高效分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：6ketoPGF1a
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：50 pg/mL-800 pg/mL
灵敏度：50 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 8000.2180.2110.215 4000.3500.3330.342 2000.6030.6120.608 1001.1111.1021.107 502.2192.1782.199 | ||||||
货期：3-5 working days
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