首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠5核苷酸酶(5-NT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5-NT含量。5-NT即5’-核苷酸酶，它能特异性水解核苷酸。其作为靶点有重要意义，在肿瘤等疾病发生发展中发挥作用。研究机制上，主要是围绕其活性调节、与底物结合模式等方面展开，期望通过调控它来干预相关生理病理过程。试剂盒检测范围为0.125 mU/mL-8 mU/mL，适用于科研领域探究代谢性疾病模型、免疫调节机制或药物干预效果等研究，为实验室研究能量代谢调控、器官功能评估及疾病机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
缩写：5-NT
Uniprot No.：Q61503
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.125 mU/mL-8 mU/mL
灵敏度：0.031 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 83 | |||||
| Range % | 80-85 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
回收率：
| The recovery of mouse 5-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-90 | |||||
| EDTA plasma (n=4) | 100 | 95-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected82.0571.9682.0131.86041.3971.4021.4001.24720.9210.9000.9110.75810.5750.6010.5880.4350.50.3670.3700.3690.2160.250.2550.2610.2580.1050.1250.1970.1900.1940.04100.1500.1560.153 | |||||||
货期：3-5 working days
×
