首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠5羟色胺(5-HT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5HT含量。5-HT即血清素，是一种重要的神经递质。它广泛分布于中枢神经系统与外周组织。在研究机制上，参与调节情绪、睡眠、食欲等多种生理功能，异常水平与抑郁症等精神疾病相关。当前围绕其开展的研究，旨在开发相关药物来治疗各类身心疾病。试剂盒检测范围为250 pg/mL-4000 pg/ml，该产品适用于神经科学研究中的递质动态分析、药理学实验的干预效果评估、行为学模型验证以及代谢相关疾病动物模型的机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5HT
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：250 pg/mL-4000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 90-89 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 40000.1300.1200.125 20000.2430.2410.242 10000.4520.4190.436 5000.7920.8310.812 2501.0681.1201.094 02.1512.0262.089 | ||||||
货期：3-5 working days
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