首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人5羟色胺(5-HT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5HT含量。5HT即血清素，是一种重要的神经递质。其在调节情绪、睡眠、食欲等方面发挥关键作用。研究机制上，主要通过与不同受体结合，影响神经信号传导来调节生理和心理功能，对其研究有助于揭示相关疾病发病机制及开发新药。试剂盒检测范围为4 ng/mL-800 ng/mL，特别适用于神经递质代谢研究、精神疾病动物模型分析、药理学实验中的药效评估等科研领域，例如探究抑郁症或肠易激综合征模型中5-HT的动态变化，或筛选影响5-HT合成/代谢的化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5HT
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4 ng/mL-800 ng/mL
灵敏度：2 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 8000.2150.2160.216 2000.3830.3910.387 640.5750.5670.571 160.9860.9740.980 41.5711.4721.522 02.4442.3452.395 | ||||||
货期：3-5 working days
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