首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠5羟色胺(5-HT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的5HT含量。5HT，即5-羟色胺，是一种重要的神经递质，广泛存在于中枢神经系统和胃肠道中。其研究机制主要涉及5-HT受体的不同亚型及其在调节胃肠道运动、感觉、分泌等功能中的作用，以及与多种胃肠道疾病的关系。通过研究5-HT信号系统，有助于了解胃肠道疾病的发病机制，并为治疗提供新的思路。试剂盒检测范围为0.8 ng/mL-160 ng/mL，适用于神经生物学、药理学及代谢疾病等领域的科学研究，例如探究药物干预、应激反应或基因修饰对大鼠模型5-HT分泌的影响，亦可评估脑组织、肠道等器官中5-HT的分布特征。为探索5-HT在神经信号传递、行为调控及病理生理机制中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5HT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.8 ng/mL-160 ng/mL
灵敏度：0.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 89-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 96 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 1600.0690.0740.072 400.1810.1950.188 12.80.4260.4390.433 3.20.8020.8550.829 0.81.1551.2311.193 02.1582.2272.193 | ||||||
本试剂盒所含材料：
A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit 5HT antibody.
Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Anti-rat 5HT Antibody 1 x 6 ml -- Act as the detection antibody.
HRP-conjugated 5HT 1 x 6 ml -- Bind to the 5HT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
