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产品描述：人5羟二十碳四烯酸(5-HETE)酶联免疫试剂盒检测血清、血浆样本中的5-HETE含量。5-HETE即5-羟二十碳四烯酸，是花生四烯酸经5-脂氧合酶代谢的产物。它在炎症、过敏等病理过程中扮演重要角色。研究机制主要围绕其在细胞信号传导中的作用，能调节免疫细胞活性、促进血管生成等，是药物研发潜在靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于体外定量分析生物样本中5-HETE的表达水平，为研究炎症相关疾病机制、评估药物干预效果或探索代谢通路调控提供可靠的实验工具，广泛应用于生命科学基础研究、药理学研究及生物标志物开发等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5-HETE
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 89 | |
| Range % | 84-94 | ||
| 1:200 | Average % | 92 | |
| Range % | 87-99 | ||
| 1:400 | Average % | 93 | |
| Range % | 85-97 | ||
| 1:800 | Average % | 97 | |
| Range % | 90-104 |
回收率：
| The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 91 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.7392.6522.6962.586 10002.2582.1762.2172.107 5001.5131.5041.5091.399 2500.8050.7420.7740.664 1250.4340.4130.4240.314 62.50.2750.2610.2680.158 31.250.1820.1790.1810.071 00.1120.1070.110 | ||||||
货期：3-5 working days
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