首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠5羟基吲哚乙酸(5-HIAA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5-HIAA含量。5-HIAA即5-羟吲哚乙酸，是血清素的主要代谢产物。它在神经生物学研究中是重要指标，可反映血清素的代谢水平。其研究机制主要围绕血清素能神经功能展开，通过检测体内含量变化，辅助探究神经系统疾病如抑郁、焦虑等发病机制。试剂盒检测范围为25 ng/mL-400 ng/mL，常用于神经递质代谢研究、神经退行性疾病模型评估以及精神类药物作用机制探索等领域。适用于实验室开展神经生物学、药理学或毒理学研究，例如评估药物干预对5 - 羟色胺代谢通路的影响，或探究特定病理状态下神经递质动态变化等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5-HIAA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：25 ng/mL-400 ng/mL
灵敏度：25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HIAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of rat 5-HIAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 94 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 4000.2830.2780.281 2000.5400.5270.534 1000.8340.9010.868 501.6981.6761.687 252.6162.5242.570 | ||||||
货期：3-5 working days
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