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产品描述：人Ⅰ型胶原交联羧基末端肽(CTX-I)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTX-I含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，常用于骨质疏松机制研究、骨关节疾病模型评估及抗骨吸收药物开发等科研领域；适用于体外骨代谢标志物检测、细胞模型骨吸收活性分析及动物实验样本评估等非临床研究场景，为骨生物学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
缩写：CTX-I
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-92 | ||||
| EDTA plasma (n=4) | 96 | 93-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.9952.9602.9782.838 202.5572.5702.5642.424 101.6661.7651.7161.576 50.8390.8860.8630.723 2.50.4910.5240.5080.368 1.250.2630.2510.2570.117 0.6250.2010.2120.2070.067 00.1380.1420.140 | ||||||
货期：3-5 working days
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