Mouse FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit

中文名： 小鼠FMS样酪氨酸激酶3配体(Flt3L)ELISA Kit

货号: IPD-E04555m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠FMS样酪氨酸激酶3配体(Flt3L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FLT3LG含量。FLT3LG是一种细胞因子，由FLT3LG基因编码，主要作用是促进造血干细胞及其祖细胞的再生和分化。FLT3LG通过激活FLT3受体，触发下游信号通路，如PI3K/AKT和Ras/MAPK，从而启动细胞生长和分化。FLT3LG在免疫系统中也扮演重要角色，特别是对树突状细胞的发育具有调控作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于免疫调节机制研究、造血系统疾病模型评估、肿瘤微环境分析等科研领域，支持通过检测不同生理或病理状态下生物样本的Flt3L表达水平，为免疫调控相关的基础研究及药物开发提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Flt3lg; Flt3l; Fms-related tyrosine kinase 3 ligand; Flt3 ligand; Flt3L; SL cytokine
缩写：
FLT3LG
Uniprot No.：
P49772
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)126.466124.651125.202125.967124.971126.858SD0.0280.0300.0270.0350.0370.034CV(%)5.2345.6615.0806.5596.9716.342

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Flt-3L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

105

Range %

97-110

1:2

Average %

101

Range %

96-105

1:4

Average %

Range %

91-100

1:8

Average %

Range %

88-99

回收率：

The recovery of mouse Flt-3L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

103

95-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












pg/ml	OD1	OD2	Average	Corrected
1000	2.233	2.356	2.294	2.138
500	1.422	1.414	1.418	1.262
250	0.888	0.856	0.872	0.716
125	0.583	0.540	0.561	0.405
62.5	0.339	0.334	0.337	0.181
31.2	0.271	0.257	0.264	0.108
15.6	0.199	0.188	0.193	0.037
0	0.154	0.158	0.156