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Human FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit

  • 中文名： 人FMS样酪氨酸激酶3配体(Flt-3L)ELISA Kit
货号: IPD-E04554h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人FMS样酪氨酸激酶3配体(Flt-3L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FLT3LG含量。FLT3LG即FMS样酪氨酸激酶3配体，是促进造血干细胞增殖和分化的细胞因子。其研究机制主要围绕与受体结合后激活下游信号通路，在免疫治疗、血液学等领域被深入研究，有望为相关疾病如白血病等的治疗带来新策略。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。适用于体外检测生物样本中Flt-3L的表达水平，可支持血液疾病研究、免疫调节机制探索、干细胞分化相关实验及炎症或肿瘤微环境等基础科研领域的数据获取，为相关领域的分子机制研究提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：FL ELISA Kit; Flt 3 ligand ELISA Kit; Flt 3L ELISA Kit; Flt3 L ELISA Kit; FLT3 LG ELISA Kit; Flt3 ligand ELISA Kit; Flt3L ELISA Kit; FLT3L_HUMAN ELISA Kit; Flt3lg ELISA Kit; Fms related tyrosine kinase 3 ligand ELISA Kit; Fms-related tyrosine kinase 3 ligand ELISA Kit; SL cytokine ELISA Kit

  • 缩写：FLT3LG

  • Uniprot No.：P49771

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma,tissue homogenates

  • 检测范围：7.8 pg/mL-500 pg/mL

  • 灵敏度：1.95 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Flt-3L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.




    		SampleSerum(n=4)

    1:1Average %92

    Range %88-95


    1:2Average %98

    Range %91-103


    1:4Average %92

    Range %85-97


    1:8Average %102

    Range %91-107


  • 回收率：

    The recovery of human Flt-3L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8682-91

    EDTA plasma (n=4)9994-105

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected  40002.0432.0002.0221.889  20001.5221.4681.4951.362  10000.9580.9270.9430.810  5000.6220.6010.6120.479  2500.4370.4560.4470.314  1250.3010.2950.2980.165  62.50.2370.2310.2340.101  00.1350.1310.133　  







  • 货期：3-5 working days

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