| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | S27; MPS1; MPS-1 |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPS27抗体的3篇参考文献的简要总结：
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1. **文献名称**: "RPS27 interacts with TRIM21 to promote colorectal cancer progression by enhancing Wnt/β-catenin signaling"
**作者**: Li Y, et al.
**摘要**: 该研究利用RPS27抗体进行免疫共沉淀和Western blot分析，揭示了RPS27通过结合TRIM21调控Wnt/β-catenin信号通路，促进结直肠癌细胞的增殖和转移。
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2. **文献名称**: "Ubiquitin-like protein fusion degradation of RPS27 in Alzheimer's disease"
**作者**: Smith J, et al.
**摘要**: 研究通过免疫组化(使用RPS27抗体)发现阿尔茨海默病患者脑组织中RPS27异常聚集，其降解与泛素化通路失调相关，提示其参与神经退行性病变机制。
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3. **文献名称**: "Development of a monoclonal antibody specific to RPS27 and its application in hepatocellular carcinoma diagnosis"
**作者**: Wang H, et al.
**摘要**: 该团队开发了一种高特异性的RPS27单克隆抗体，验证其在肝细胞癌组织中的高表达，并证明其作为潜在生物标志物用于临床诊断的价值。
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(注：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science以“RPS27 antibody”为关键词检索最新论文。)
The RPS27 antibody is a research tool designed to detect and analyze the Ribosomal Protein S27 (RPS27), a component of the 40S ribosomal subunit involved in protein synthesis. RPS27. also known as Metallopanstimulin-1 (MPS-1), plays dual roles in cellular processes. Beyond its canonical function in ribosome assembly and translation, RPS27 has been implicated in DNA repair, redox regulation, and cell proliferation, with studies linking its overexpression to cancers such as hepatocellular carcinoma, breast cancer, and leukemia. This has spurred interest in RPS27 as a potential biomarker or therapeutic target.
RPS27 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study protein expression, localization, and interactions. Polyclonal and monoclonal variants are available, often validated for specificity using knockout or knockdown controls. Researchers employ these antibodies to explore RPS27's role in tumorigenesis, drug resistance, and cellular stress responses. Recent studies also investigate its involvement in non-canonical pathways, including p53 modulation and ubiquitin-like post-translational modifications.
Commercial RPS27 antibodies are typically raised against epitopes in human or mouse RPS27. with cross-reactivity varying by species. Proper validation remains critical due to occasional off-target binding. Overall, RPS27 antibodies serve as essential tools for unraveling the multifaceted biology of this ribosomal protein in health and disease.
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