| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GDI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于GDI1相关研究领域构造的模拟参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**: "Characterization of GDI1 mutations in X-linked intellectual disability using specific antibodies"
**作者**: Smith A, et al.
**摘要**: 本研究利用抗GDI1抗体分析X连锁智力障碍患者的突变蛋白表达，发现特定突变导致GDI1与Rab GTPases的结合能力下降，影响神经元囊泡运输。
2. **文献名称**: "Structural insights into GDI1-Rab complex regulation by cryo-EM"
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜解析GDI1-Rab复合物结构，结合抗体标记技术，揭示GDI1调控Rab蛋白膜循环的分子机制，为相关疾病治疗提供新靶点。
3. **文献名称**: "GDI1 antibody-based detection of dysregulated synaptic vesicles in Alzheimer's models"
**作者**: Wang Y, et al.
**摘要**: 采用抗GDI1抗体检测阿尔茨海默病模型小鼠脑组织，发现GDI1表达异常与突触囊泡聚集缺陷相关，提示其参与神经退行性病理过程。
4. **文献名称**: "Development of a high-affinity monoclonal antibody for GDI1 functional studies"
**作者**: Tanaka K, et al.
**摘要**: 报道一种新型单克隆抗体的开发，验证其对GDI1的特异性识别，并应用于免疫共沉淀实验，证实GDI1在癌细胞迁移中的调控作用。
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**注意**：以上内容为模拟示例，实际文献请通过 **PubMed/Google Scholar** 等平台以关键词 "GDI1 antibody" 或结合具体研究领域(如神经科学、癌症)检索。
The GDP dissociation inhibitor 1 (GDI1) antibody is a tool used to study the GDI1 protein, which belongs to the Rab GDP-dissociation inhibitor (GDI) family. GDI1 regulates Rab GTPases, key players in intracellular vesicle trafficking. By binding to Rab proteins in their inactive GDP-bound state, GDI1 prevents nucleotide exchange and ensures their proper recycling between membranes and the cytosol. This function is critical for maintaining vesicle transport, organelle organization, and membrane dynamics in eukaryotic cells.
GDI1 antibodies are widely employed in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They have been instrumental in studies linking GDI1 dysfunction to neurological disorders, particularly X-linked intellectual disability syndrome (XLID) caused by GDI1 mutations. Additionally, altered Rab-GDI pathways are implicated in cancers and neurodegenerative diseases like Alzheimer’s, making these antibodies valuable for exploring disease mechanisms.
Commercially available GDI1 antibodies are typically raised against specific epitopes, with validation data confirming reactivity across human, mouse, and rat samples. Researchers rely on them to dissect GDI1’s role in cellular homeostasis and pathology, bridging molecular insights with therapeutic development.
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