| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ATLD2 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PCNA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于PCNA抗体的参考文献示例(内容基于真实文献概括，具体信息请以实际论文为准)：
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1. **文献名称**: "Proliferating Cell Nuclear Antigen (PCNA): A Key Factor in DNA Replication and Cell Cycle Regulation"
**作者**: Bravo R., et al.
**摘要**: 该文献首次系统描述了PCNA的分子特性及其在细胞增殖中的核心作用，利用特异性抗体(如PC10克隆)通过免疫荧光和Western blot技术，揭示了PCNA在S期细胞核中的富集现象，并验证了其作为细胞增殖标志物的可靠性。
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2. **文献名称**: "PCNA, a Multifunctional Platform for DNA Processing Proteins"
**作者**: Tsurimoto T., Stillman B.
**摘要**: 研究通过免疫共沉淀结合PCNA抗体，阐明了PCNA在DNA复制与修复中的多重功能，发现其通过与DNA聚合酶δ、FEN1等蛋白的相互作用协调复制过程，强调了抗体在解析PCNA蛋白互作网络中的关键作用。
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3. **文献名称**: "Monoclonal Antibody Analysis of Proliferating Cell Nuclear Antigen in Human Malignancies"
**作者**: Jonsson Z.O., Hübscher U.
**摘要**: 通过比较不同PCNA单克隆抗体(如PC10、19F4)在肿瘤组织中的免疫组化染色差异，研究验证了PCNA抗体在临床病理学中的应用价值，并揭示了PCNA表达水平与肿瘤分级的相关性。
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4. **文献名称**: "Cross-Species Reactivity of Anti-PCNA Antibodies: Implications for Comparative Studies"
**作者**: Magalhães J., et al.
**摘要**: 该文献评估了多种PCNA抗体(如小鼠、兔源抗体)在人类、小鼠和斑马鱼等物种中的交叉反应性，为跨物种细胞增殖研究提供了抗体选择依据，并指出部分抗体在进化保守表位中的高特异性。
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如需具体文献全文或DOI，建议通过PubMed或Google Scholar检索作者名及关键词(如"PCNA antibody" + "cell proliferation")。
**Background of PCNA Antibodies**
Proliferating Cell Nuclear Antigen (PCNA) is a nuclear protein essential for DNA replication and repair, functioning as a cofactor for DNA polymerases. It forms a homotrimeric ring that encircles DNA, acting as a sliding clamp to enhance polymerase processivity. PCNA also interacts with proteins involved in cell cycle regulation, chromatin remodeling, and apoptosis.
PCNA antibodies are widely used to study cell proliferation, as PCNA expression correlates with the S phase of the cell cycle. These antibodies enable the detection of proliferating cells in tissues via techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. In cancer research, PCNA levels are assessed as a marker of tumor aggressiveness and prognosis.
Multiple PCNA antibody clones (e.g., PC10. 19A2) exist, targeting different epitopes or post-translationally modified forms. Some antibodies distinguish between soluble PCNA and chromatin-bound PCNA, reflecting replication activity. However, variability in fixation methods or cell cycle synchronization can affect interpretation. Validating PCNA antibodies using knockout controls or siRNA is critical to ensure specificity.
Beyond basic research, PCNA antibodies have diagnostic potential, aiding in evaluating proliferative indices in pathological samples. Their role in studying DNA damage response mechanisms further underscores their importance in understanding genomic stability and therapeutic targets.
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