| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HNRPU; SAF-A; U21.1; hnRNP U |
| Entrez GeneID | 3192 |
| clone | 1C5H11 |
| WB Predicted band size | 90kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HNRNPU expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HNRNPU抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*HNRNPU regulates the tumorigenic potential of glioblastoma by regulating cell cycle progression*
**作者**：Li X, et al.
**摘要**：该研究利用HNRNPU抗体进行免疫沉淀和Western blot分析，发现HNRNPU通过调控细胞周期相关基因(如Cyclin D1)的表达，影响胶质母细胞瘤的增殖和侵袭能力，提示其作为潜在治疗靶点。
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2. **文献名称**：*A novel HNRNPU antibody reveals its role in neuronal differentiation and DNA damage response*
**作者**：Wang Y, et al.
**摘要**：研究开发了一种高特异性HNRNPU抗体，验证其在免疫荧光和流式细胞术中的应用，发现HNRNPU缺失会阻碍神经元分化并增强DNA损伤应答，可能与神经发育疾病相关。
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3. **文献名称**：*HNRNPU interacts with chromatin remodeling complexes to modulate gene expression in autism spectrum disorder*
**作者**：Sanders SJ, et al.
**摘要**：通过ChIP-seq结合HNRNPU抗体，研究发现HNRNPU与染色质重塑复合物(如SWI/SNF)相互作用，调控自闭症相关基因的表达，揭示了其在神经发育中的表观遗传调控机制。
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(注：上述文献为示例，实际引用时需核实具体来源及细节。)
The HNRNPU antibody targets the heterogeneous nuclear ribonucleoprotein U (hnRNPU), a multifunctional RNA-binding protein involved in pre-mRNA splicing, transcriptional regulation, chromatin remodeling, and genomic stability. Also known as SAF-A (scaffold attachment factor A) or SP120. HNRNPU plays critical roles in neuronal development, cell cycle control, and DNA repair. Mutations in the HNRNPU gene are linked to neurodevelopmental disorders, including intellectual disability and epilepsy, as well as cancers such as hepatocellular carcinoma and leukemia.
HNRNPU antibodies are widely used in research to study its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). These antibodies help elucidate HNRNPU's role in RNA metabolism, chromatin organization, and disease mechanisms. Commercial HNRNPU antibodies are typically raised against specific epitopes, with monoclonal and polyclonal variants available. Validation data, including knockout cell line controls, are essential to confirm specificity due to potential cross-reactivity with other hnRNP family members.
Research applications often focus on HNRNPU's involvement in regulating alternative splicing, X-chromosome inactivation, and stress granule formation. Its dynamic shuttling between nucleus and cytoplasm under stress conditions makes it a key subject in studies on cellular stress responses and neurodegeneration.
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