| WB | 1/500 - 1/2000 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 咨询技术 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/10000 | Human,Mouse,Monkey |
| Aliases | AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; AKT1 |
| Entrez GeneID | 207 |
| clone | 3A3 |
| WB Predicted band size | 56kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Purified recombinant fragment of human AKT1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于AKT1抗体的3篇参考文献示例(基于已知研究领域概括，非真实文献)：
1. **文献名称**：*AKT1-specific monoclonal antibody inhibits tumor growth in PTEN-deficient cancers*
**作者**：Chen et al.
**摘要**：本研究开发了一种高特异性AKT1单克隆抗体，通过靶向AKT1的磷酸化位点抑制其活性，在PTEN缺失的肿瘤模型中显著抑制肿瘤增殖并诱导凋亡。
2. **文献名称**：*Validation of a phospho-specific AKT1 antibody for immunohistochemical analysis in breast cancer*
**作者**：Smith et al.
**摘要**：文章验证了一种磷酸化AKT1(Ser473)抗体的特异性，证明其在乳腺癌组织中的可靠检测能力，并揭示其表达水平与患者预后不良相关。
3. **文献名称**：*AKT1 antibody-based biosensor for real-time monitoring of kinase activity in cells*
**作者**：Kim et al.
**摘要**：研究利用AKT1抗体构建新型生物传感器，成功实时监测活细胞内AKT1激酶动态变化，为靶向药物筛选提供新工具。
4. **文献名称**：*Cross-reactivity analysis of commercial AKT1 antibodies reveals variability in isoform specificity*
**作者**：Johnson et al.
**摘要**：通过评估市售AKT1抗体的交叉反应性，发现不同抗体对AKT1同源异构体(如AKT2/AKT3)的特异性差异，提示实验设计中需谨慎选择验证抗体。
(注：以上文献为虚拟示例，实际引用时需查询真实数据库如PubMed并核实信息。)
The AKT1 antibody is a crucial tool in studying the AKT serine/threonine kinase 1. a key player in the PI3K-AKT-mTOR signaling pathway. AKT1. a proto-oncogene, regulates cellular processes including survival, proliferation, metabolism, and angiogenesis. It contains a PH domain for membrane binding and a kinase domain activated by phosphorylation at Thr308 (by PDK1) and Ser473 (by mTORC2). Dysregulated AKT1 is linked to cancers, diabetes, and neurological disorders, making it a therapeutic target.
AKT1 antibodies detect total AKT1 protein or specific phosphorylation states, enabling research into pathway activation status. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze expression patterns in tissues or cell lines. These antibodies help validate AKT1 inhibition in drug development studies and assess biomarker potential in cancer diagnostics. Both monoclonal and polyclonal variants exist, with phosphorylation-specific antibodies being critical for functional studies. Their applications span basic research, preclinical drug evaluation, and clinical diagnostics, underscoring AKT1's central role in cellular signaling and disease pathogenesis.
×
