| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CATX-8; RAB25 |
| Entrez GeneID | 57111 |
| clone | 3F12 |
| WB Predicted band size | 24kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of Rab25 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Rab25抗体的参考文献示例(注：以下内容为模拟示例，仅供参考)：
1. **文献名称**：Rab25在卵巢癌中的表达及其临床意义
**作者**：Cheng, J.M., et al.
**摘要**：该研究通过免疫组化分析发现Rab25在卵巢癌组织中高表达，且与肿瘤转移及患者预后不良显著相关，提示Rab25可能作为卵巢癌的生物标志物。
2. **文献名称**：Rab25通过调节整合素β1运输促进肿瘤细胞侵袭
**作者**：Zhang, Y., et al.
**摘要**：研究利用Rab25抗体进行蛋白质互作实验，揭示Rab25通过调控整合素β1的细胞膜定位，增强肿瘤细胞的迁移和侵袭能力，为靶向治疗提供依据。
3. **文献名称**：Rab25在结直肠癌中的功能及其分子机制
**作者**：Wang, L., et al.
**摘要**：通过敲低Rab25表达并结合抗体检测，证实Rab25通过激活PI3K/AKT信号通路促进结直肠癌细胞增殖，并抑制凋亡。
4. **文献名称**：Rab25作为胃癌早期诊断标志物的研究
**作者**：Liu, H., et al.
**摘要**：利用Rab25抗体检测胃癌患者血清外泌体，发现Rab25水平显著升高，提示其可能作为无创诊断胃癌的潜在标志物。
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**注意**：以上文献信息为示例，实际引用时请通过学术数据库(如PubMed、Web of Science)检索真实文献并核对信息。
Rab25. a member of the RAS oncogene family, is a small GTPase involved in vesicle trafficking and membrane transport processes. It regulates apical recycling endosomes, influencing cell polarity, migration, and signal transduction. Rab25 has gained attention for its dual role in cancer progression. While overexpressed in some cancers (e.g., ovarian, breast, and colon cancers), where it promotes tumor aggressiveness and chemoresistance, it acts as a tumor suppressor in others (e.g., certain gastric and prostate cancers). This context-dependent behavior makes Rab25 a complex biomarker and therapeutic target.
Rab25 antibodies are critical tools for studying its expression patterns, intracellular localization, and functional mechanisms. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercially available antibodies are often developed in rabbits or mice, targeting specific epitopes of human Rab25. Validation includes testing in knockdown models (siRNA/CRISPR) to confirm specificity, as cross-reactivity with other Rab family members is a common concern.
Research using these antibodies has linked Rab25 to key pathways, including PI3K/AKT and integrin signaling, offering insights into metastasis and drug resistance. Clinically, Rab25 antibody-based assays are explored for prognostic stratification. However, standardization of antibody protocols remains challenging due to variability in tumor microenvironments and post-translational modifications of Rab25. Ongoing studies aim to refine antibody specificity and expand applications in diagnostics and targeted therapies.
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