| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUFM |
| Uniprot No | P49411 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-452aa |
| 氨基酸序列 | AVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYE EIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAP LDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVE LVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAV DTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELL GHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKP GSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPP EKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMT EEEKNIKWG |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TUFM重组蛋白研究的示例参考文献(内容为虚构，仅作格式参考)：
1. **"Recombinant expression and functional characterization of mitochondrial elongation factor Tu (TUFM) in human cell lines"**
*作者：Li, X., et al.*
摘要：研究通过哺乳动物表达系统(HEK293细胞)重组表达TUFM蛋白，证实其在线粒体翻译中的关键作用，并发现过表达TUFM可抑制ROS生成，减轻细胞氧化应激损伤。
2. **"Crystal structure analysis of TUFM recombinant protein from E. coli expression system"**
*作者：Zhang, Y., & Wang, H.*
摘要：报道利用大肠杆菌系统高效表达并纯化TUFM重组蛋白，通过X射线晶体学解析其三维结构，揭示了其与线粒体RNA结合的分子机制。
3. **"TUFM recombinant protein promotes apoptosis via modulating mTOR signaling in colorectal cancer"**
*作者：Chen, L., et al.*
摘要：研究发现重组TUFM蛋白在结直肠癌细胞中通过调控mTOR通路诱导凋亡，为靶向TUFM的癌症治疗提供了实验依据。
如需真实文献，建议在PubMed或Google Scholar中检索关键词：**TUFM recombinant protein expression** 或 **TUFM mitochondrial elongation factor purification**。
**Background of TUFM Recombinant Protein**
TUFM (Mitochondrial Tu Translation Elongation Factor), also known as EF-TuMT, is a nuclear-encoded protein critical for mitochondrial translation and energy metabolism. It functions as a GTPase that facilitates the elongation phase of protein synthesis within mitochondria by delivering aminoacyl-tRNAs to the ribosome. Beyond its canonical role, TUFM has emerged as a multifunctional regulator implicated in cellular processes such as autophagy, innate immunity, and apoptosis. Dysregulation of TUFM is linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s), and cancer, highlighting its broad physiological and pathological relevance.
Recombinant TUFM protein is engineered using genetic cloning techniques, where the *TUFM* gene is expressed in heterologous systems like *E. coli* or mammalian cells. This allows large-scale production of purified, bioactive TUFM for research and therapeutic applications. The recombinant form retains functional domains essential for GTP binding, tRNA interactions, and mitochondrial localization. Its availability enables mechanistic studies, such as elucidating TUFM’s role in stress responses (e.g., mediating Parkin-dependent mitophagy) or its interplay with signaling pathways like mTOR and AMPK.
In drug discovery, recombinant TUFM serves as a tool for screening compounds targeting mitochondrial dysfunction or cancer metabolism. Structural studies using X-ray crystallography or cryo-EM further leverage recombinant TUFM to resolve molecular mechanisms and guide rational drug design. Overall, TUFM recombinant protein bridges basic mitochondrial biology with translational research, offering insights into disease mechanisms and therapeutic strategies.
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