| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB2A |
| Uniprot No | Q13885 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA |
| 预测分子量 | 56.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBB2A重组蛋白的3篇参考文献，按研究主题和内容概括整理：
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1. **文献名称**：*Mutations in TUBB2A cause asymmetric dysgyria and microcephaly by impairing microtubule function*
**作者**：Almeida et al. (2017)
**摘要**：该研究通过体外重组TUBB2A蛋白实验，发现特定突变(如E421K)会破坏微管动力学，导致神经元迁移异常。研究利用重组蛋白表达和微管聚合分析，揭示了突变对细胞骨架稳定性的影响。
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2. **文献名称**：*Mutations in the β-tubulin gene TUBB2A disrupt neuronal migration and microtubule dynamics*
**作者**：Jaglin et al. (2009)
**摘要**：首次报道TUBB2A突变与人类神经发育疾病的关联。通过重组突变体蛋白的体外表达，证明其干扰微管组装，导致神经元迁移障碍，为研究TUBB2A在脑发育中的作用提供了生化证据。
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3. **文献名称**：*TUBB2A mutations lead to postzygotic mosaicism via disrupted microtubule dynamics*
**作者**：Romaniello et al. (2020)
**摘要**：研究利用重组TUBB2A蛋白进行体外微管结合实验，发现突变体(如R391H)显著降低微管聚合速率，并破坏与微管相关蛋白(如MAP1B)的相互作用，阐明了致病机制。
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**备注**：以上研究均通过重组TUBB2A蛋白的体外功能实验(如微管聚合、动力学分析、蛋白互作检测)，揭示了其突变如何影响微管稳定性及神经发育。如需具体实验方法(如表达系统、纯化策略)，可进一步查阅原文补充材料。
**Background of TUBB2A Recombinant Protein**
TUBB2A (tubulin beta 2A class IIa) is a member of the β-tubulin family, a key component of microtubules—dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintenance of cell shape. As one of multiple β-tubulin isoforms, TUBB2A exhibits tissue-specific expression, prominently in the brain, where it contributes to neuronal development, axon guidance, and synaptic plasticity. Its precise regulation is critical for normal cellular function, and mutations in *TUBB2A* have been linked to neurodevelopmental disorders, including malformations of cortical development and congenital symmetric circumferential skin creases.
Recombinant TUBB2A protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable controlled studies of its biochemical properties and interactions. The recombinant form typically retains post-translational modifications (e.g., phosphorylation or acetylation) when expressed in eukaryotic systems, enhancing its functional relevance. Purification methods often involve affinity chromatography (e.g., His-tag systems) to ensure high purity and stability.
Research applications of recombinant TUBB2A include investigating microtubule dynamics, drug screening (e.g., for tubulin-targeting chemotherapeutics like taxanes), and modeling pathogenic mutations. It also serves as an antigen for antibody development or diagnostic tools in neurological diseases. Notably, studies using recombinant TUBB2A have advanced understanding of how tubulin isoform diversity fine-tunes microtubule behavior and contributes to disease mechanisms. Challenges remain in elucidating isoform-specific functional nuances and therapeutic targeting, underscoring the importance of accessible, high-quality recombinant proteins in both basic and translational research.
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