| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RO60 |
| Uniprot No | P10155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-535aa |
| 氨基酸序列 | ESVNQMQPLNEKQIANSQDGYVWQVTDMNRLHRFLCFGSEGGTYYIKEQKLGLENAEALIRLIEDGRGCEVIQEIKSFSQEGRTTKQEPMLFALAICSQCSDISTKQAAFKAVSEVCRIPTHLFTFIQFKKDLKESMKCGMWGRALRKAIADWYNEKGGMALALAVTKYKQRNGWSHKDLLRLSHLKPSSEGLAIVTKYITKGWKEVHELYKEKALSVETEKLLKYLEAVEKVKRTRDELEVIHLIEEHRLVREHLLTNHLKSKEVWKALLQEMPLTALLRNLGKMTANSVLEPGNSEVSLVCEKLCNEKLLKKARIHPFHILIALETYKTGHGLRGKLKWRPDEEILKALDAAFYKTFKTVEPTGKRFLLAVDVSASMNQRVLGSILNASTVAAAMCMVVTRTEKDSYVVAFSDEMVPCPVTTDMTLQQVLMAMSQIPAGGTDCSLPMIWAQKTNTPADVFIVFTDNETFAGGVHPAIALREYRKKMDIPAKLIVCGMTSNGFTIADPDDRGMLDMCGFDTGALDVIRNFTL |
| 预测分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RO60重组蛋白的3篇参考文献概览(虚构示例，仅供参考格式)：
1. **《Structural insights into RO60 autoantigen recognition by RNA》**
- 作者：Smith A, et al.
- 摘要：通过X射线晶体学解析RO60蛋白与Y RNA复合物的结构，揭示其结合RNA的关键结构域，阐述其在自身免疫疾病中作为抗原的潜在机制。
2. **《Recombinant RO60 expression and its role in systemic lupus erythematosus》**
- 作者：Chen L, et al.
- 摘要：构建RO60重组蛋白表达系统，发现其与患者血清中自身抗体的强反应性，提示RO60在系统性红斑狼疮诊断中的生物标志物潜力。
3. **《RO60 interacts with the RNA exosome to regulate viral RNA decay》**
- 作者：Wang Y, et al.
- 摘要：研究重组RO60蛋白与RNA外泌体的相互作用，证明其在宿主抗病毒免疫中通过降解病毒RNA的分子机制。
注：以上文献为示例，实际引用需根据具体研究领域检索真实数据库(如PubMed)。建议使用关键词“RO60 recombinant protein”或“SSB/La protein”查找近期论文。
RO60. also known as SS-A (Sjögren's syndrome antigen A), is a highly conserved RNA-binding protein encoded by the TROVE2 gene. It plays a critical role in RNA metabolism, quality control, and cellular stress responses. Structurally, RO60 forms a ring-shaped complex with non-coding RNAs called Y RNAs, functioning as part of ribonucleoprotein (RNP) particles. This interaction is essential for regulating RNA stability and preventing immune recognition of self-nucleic acids.
In autoimmune diseases like systemic lupus erythematosus (SLE) and Sjögren's syndrome, RO60 becomes a major autoantigen. Autoantibodies against RO60 are clinically significant biomarkers for diagnosis and disease monitoring. Research suggests that aberrant exposure of RO60-RNA complexes during cell apoptosis may trigger loss of immune tolerance, leading to pathogenic autoantibody production.
Recombinant RO60 protein is produced using genetic engineering techniques, typically through bacterial (e.g., E. coli) or eukaryotic expression systems. This engineered protein retains key epitopes for antibody recognition while eliminating contamination risks associated with native tissue extraction. Its applications span autoimmune disease research, diagnostic kit development (e.g., ELISA), and studies of RNA-protein interactions. Recent investigations also explore its role in cellular responses to UV radiation, viral infections, and cancer biology. As an essential tool in autoimmunity studies, recombinant RO60 continues to advance our understanding of molecular mechanisms in rheumatic diseases and potential therapeutic interventions.
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