| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCDC2 |
| Uniprot No | Q9UHG0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MSGSSARSSH LSQPVVKSVL VYRNGDPFYA GRRVVIHEKK VSSFEVFLKE VTGGVQAPFG AVRNIYTPRT GHRIRKLDQI QSGGNYVAGG QEAFKKLNYL DIGEIKKRPM EVVNTEVKPV IHSRINVSAR FRKPLQEPCT IFLIANGDLI NPASRLLIPR KTLNQWDHVL QMVTEKITLR SGAVHRLYTL EGKLVESGAE LENGQFYVAV GRDKFKKLPY SELLFDKSTM RRPFGQKASS LPPIVGSRKS KGSGNDRHSK STVGSSDNSS PQPLKRKGKK EDVNSEKLTK LKQNVKLKNS QETIPNSDEG IFKAGAERSE TRGAAEVQED EDTQVEVPVD QRPAEIVDEE EDGEKANKDA EQKEDFSGMN GDLEEEGGRE ATDAPEQVEE ILDHSEQQAR PARVNGGTDE ENGEELQQVN NELQLVLDKE RKSQGAGSGQ DEADVDPQRP PRPEVKITSP EENENNQQNK DYAAVA |
| 预测分子量 | 52,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设的DCDC2重组蛋白相关参考文献示例：
1. **《Structural analysis of DCDC2 recombinant protein in neuronal development》**
- **作者**: Smith J, et al.
- **摘要**: 研究利用大肠杆菌系统表达并纯化DCDC2重组蛋白，通过X射线晶体学解析其双皮质素结构域，揭示其与微管结合的分子机制，为神经细胞迁移障碍提供结构基础。
2. **《Functional characterization of DCDC2 mutations in dyslexia-related cellular models》**
- **作者**: Lee H, et al.
- **摘要**: 构建携带DCDC2常见突变的HEK293细胞系，发现重组蛋白的异常表达导致纤毛形成缺陷，提示DCDC2变异通过干扰神经元信号传导参与阅读障碍病理过程。
3. **《DCDC2 recombinant protein interacts with microtubule-associated proteins in vitro》**
- **作者**: Garcia R, et al.
- **摘要**: 通过Pull-down实验验证重组DCDC2蛋白与TUBB3微管蛋白的相互作用，证明其通过稳定微管网络调控皮层神经元轴突导向功能。
4. **《Expression optimization and functional screening of DCDC2 isoforms in neuronal differentiation》**
- **作者**: Wang Y, et al.
- **摘要**: 比较昆虫细胞/哺乳动物系统中DCDC2不同剪接体的重组表达效率，发现特定异构体显著促进体外培养神经前体细胞的迁移能力。
注：以上文献为假设性示例，实际研究需查询PubMed等数据库获取真实文献。
**Background of DCDC2 Recombinant Protein**
DCDC2 (Doublecortin Domain-Containing Protein 2) is a gene encoding a protein involved in neuronal migration and brain development, particularly in the formation of the cerebral cortex. It is highly expressed during early neurogenesis and plays a critical role in regulating microtubule dynamics, which are essential for proper neuronal positioning and dendritic spine formation. Mutations or dysregulation of DCDC2 have been linked to neurodevelopmental disorders, including dyslexia, autism spectrum disorders, and hearing impairments, highlighting its importance in cognitive and sensory processing.
The recombinant DCDC2 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein for research. This allows scientists to study its structural properties, interactions with cytoskeletal components (e.g., tubulin), and signaling pathways in vitro or in cellular models. Recombinant DCDC2 is particularly valuable for investigating its role in neurodevelopmental processes, such as neuronal migration defects, and for screening potential therapeutic compounds targeting DCDC2-related disorders.
Studies using DCDC2 recombinant protein have also explored its involvement in cilia function, as the protein is expressed in ciliated cells, suggesting broader implications in cellular motility and sensory perception. Additionally, its association with dyslexia has spurred research into how genetic variations in DCDC2 affect neural connectivity and language processing. By leveraging recombinant DCDC2. researchers aim to unravel molecular mechanisms underlying brain development and disease, paving the way for diagnostic or therapeutic innovations.
Overall, DCDC2 recombinant protein serves as a vital tool for bridging genetic insights with functional neurobiology, offering a deeper understanding of brain development and its disorders.
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