Recombinant Human NFX1 protein

中文名： 核转录因子X-框结合蛋白(NFX1)重组蛋白
别名： NFX1；NFX2；Transcriptional repressor NF-X1

货号: PA2000-1190

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	NFX1
Uniprot No	Q12986
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-1120aa
氨基酸序列	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD
预测分子量	124,3 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NFX1重组蛋白的3篇文献摘要概览(文献信息为模拟示例，实际需通过学术数据库验证)：

1. **文献名称**：*Functional Analysis of NFX1-123 Recombinant Protein in HPV E6-Mediated Transcriptional Regulation*

**作者**：Smith J. et al.

**摘要**：本研究通过大肠杆菌表达系统制备了重组人NFX1-123蛋白，并验证其与HPV16 E6蛋白的相互作用。实验表明，NFX1-123重组蛋白能抑制E6对端粒酶逆转录酶(hTERT)启动子的激活作用，揭示了其在HPV致癌机制中的潜在调控功能。

2. **文献名称**：*Purification and Structural Characterization of NFX1-91 Isoform for Ubiquitination Studies*

**作者**：Li R. & Wang H.

**摘要**：作者利用杆状病毒-昆虫细胞系统高效表达并纯化了NFX1-91重组蛋白，通过质谱和圆二色谱分析其结构。该蛋白被用于体外泛素化实验，证明其可作为E3泛素连接酶的底物，为研究NFX1在蛋白质降解途径中的作用提供了工具。

3. **文献名称**：*Development of a NFX1 Recombinant Protein-Based Assay for High-Throughput Drug Screening*

**作者**：Garcia M. et al.

**摘要**：研究团队开发了基于NFX1重组蛋白的荧光偏振检测方法，用于筛选调控NFX1与DNA结合活性的小分子化合物。该方法成功鉴定出两种化合物可增强NFX1对靶基因启动子的结合能力，为相关疾病治疗提供新思路。

注：以上内容为模拟生成，实际文献需通过**PubMed**或**Web of Science**以关键词“NFX1 recombinant protein”检索获取。若需具体文献，建议访问学术数据库查询最新研究。

背景信息

**Background of NFX1 Recombinant Protein**

NFX1 (Nuclear Transcription Factor, X-box Binding 1) is a protein encoded by the *NFX1* gene, which plays diverse roles in transcriptional regulation, cellular differentiation, and immune response modulation. Initially identified for its involvement in major histocompatibility complex (MHC) class II gene regulation, NFX1 interacts with conserved X-box promoter elements and collaborates with other transcription factors to modulate gene expression. It exists in multiple splice variants, with NFX1-123 and NFX1-91 being the most studied isoforms. NFX1-123. for instance, acts as a transcriptional repressor, while NFX1-91 exhibits E3 ubiquitin ligase activity, influencing protein degradation pathways.

The recombinant NFX1 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. This recombinant form retains functional domains, such as the RING finger and leucine-rich repeats, critical for its interactions and enzymatic activities. Its production enables mechanistic studies on NFX1’s role in cellular processes, including telomerase regulation, viral pathogenesis (e.g., HPV and HIV), and cancer progression. For example, NFX1 suppresses telomerase by destabilizing *hTERT* mRNA, linking it to aging and oncogenesis.

Research utilizing recombinant NFX1 has advanced understanding of its dual roles in tumor suppression and viral replication, highlighting its therapeutic potential. Structural and functional analyses of this protein also aid in developing targeted therapies for cancers and infectious diseases. Overall, NFX1 recombinant protein serves as a vital tool for dissecting molecular pathways and validating drug candidates.