| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDE1 |
| Uniprot No | Q9NZC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MWLWEDQGGLLGPFSFLLLVLLLVTRSPVNACLLTGSLFVLLRVFSFEPV PSCRALQVLKPRDRISAIAHRGGSHDAPENTLAAIRQAAKNGATGVELDI EFTSDGIPVLMHDNTVDRTTDGTGRLCDLTFEQIRKLNPAANHRLRNDFP DEKIPTLREAVAECLNHNLTIFFDVKGHAHKATEALKKMYMEFPQLYNNS VVCSFLPEVIYKMRQTDRDVITALTHRPWSLSHTGDGKPRYDTFWKHFIF VMMDILLDWSMHNILWYLCGISAFLMQKDFVSPAYLKKWSAKGIQVVGWT VNTFDEKSYYESHLGSSYITDSMVEDCEPHF |
| 预测分子量 | 37,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDE1重组蛋白的3篇参考文献，按研究方向和内容简要概括：
---
### 1. **文献名称**：*"Structural and functional characterization of glycerophosphodiester phosphodiesterase GDE1. a potential therapeutic target for neurological disorders"*
**作者**：Smith A, et al.
**摘要**：该研究解析了人源GDE1重组蛋白的晶体结构，揭示了其催化机制中关键氨基酸残基的作用。通过体外酶活实验，证明GDE1通过水解甘油磷脂信号分子调控神经元分化，为神经退行性疾病治疗提供潜在靶点。
---
### 2. **文献名称**：*"Recombinant expression and purification of human GDE1 in E. coli: Implications for lipid metabolism studies"*
**作者**：Chen L, et al.
**摘要**：研究开发了一种高效的大肠杆菌表达系统，用于生产可溶性GDE1重组蛋白。通过优化密码子和纯化步骤，获得高纯度蛋白，并验证其体外水解甘油磷脂底物的活性，为脂质代谢通路研究提供工具。
---
### 3. **文献名称**：*"GDE1 regulates oligodendrocyte differentiation through non-canonical Wnt signaling"*
**作者**：Park JH, et al.
**摘要**：利用重组GDE1蛋白进行功能实验，发现其通过抑制非经典Wnt通路促进少突胶质细胞分化。研究结合基因敲除和重组蛋白回补实验，阐明了GDE1在中枢神经系统髓鞘形成中的关键作用。
---
**注**：以上文献为示例，实际引用时需核实具体发表信息。若需扩展，可进一步检索PubMed或SciFinder数据库，关键词建议：**"GDE1 recombinant"**、**"Glycerophosphodiesterase 1 function"**。
**Background of GDE1 Recombinant Protein**
Glycerophosphodiester phosphodiesterase 1 (GDE1) is a transmembrane enzyme belonging to the glycerophosphodiester phosphodiesterase (GDPD) family. It catalyzes the hydrolysis of glycerophosphodiesters, such as glycerophosphocholine, to produce glycerol phosphate and choline phosphate, which serve as precursors for phospholipid synthesis and neurotransmitter production. GDE1 is highly expressed in the brain, kidneys, and liver, where it plays critical roles in lipid metabolism, cellular signaling, and neural development.
Research has linked GDE1 to neurodevelopmental processes, including axon guidance and synaptic plasticity, through its regulation of extracellular glycerophospholipid signaling. Additionally, GDE1 interacts with pathways involving Wnt and Notch, influencing cell differentiation and tissue homeostasis. Its dysfunction has been implicated in neurological disorders, such as Alzheimer’s disease, and metabolic conditions like non-alcoholic fatty liver disease (NAFLD).
Recombinant GDE1 protein is engineered for in vitro studies to elucidate its structural and functional properties. Produced via heterologous expression systems (e.g., mammalian or insect cells), it retains enzymatic activity and is utilized to investigate substrate specificity, inhibitor screening, and molecular interactions. Purification often involves affinity tags (e.g., His-tag) and chromatography techniques.
Recent studies highlight GDE1’s therapeutic potential. In cancer, altered GDE1 expression correlates with tumor progression, suggesting roles in metastasis or drug resistance. In regenerative medicine, GDE1-mediated lipid signaling may promote neuronal survival or stem cell differentiation. However, mechanisms remain incompletely understood, necessitating further exploration using recombinant protein tools. Overall, GDE1 represents a multifaceted enzyme with broad implications in health and disease.
×
