| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELMO3 |
| Uniprot No | Q96BJ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-720aa |
| 氨基酸序列 | MAPPRNVVKI AIKMRDAIPQ LIQLDQAKPL AAVLKEVCDA WSLTHSERYA LQFADGHRRY ITENNRAEIK NGSILCLSTA PDLEAEQLLG GLQSNSPEGR REALRRLVPL ASDMIFAREV ISRNGLQILG TIIEDGDDLG EVLALSLRAF SELMEHGVVS WETLSIPFVR KVVCYVNMNL MDASVPPLAL GLLESVTLSS PALGQLVKSE VPLDRLLVHL QVMNQQLQTK AMALLTALLQ GASPVERKHM LDYLWQRNLR QFIYKNIIHS AAPMGDEMAH HLYVLQALML GLLEPRMRTP LDPYSQEQRE QLQVLRQAAF EVEGESSGAG LSADRRRSLC AREFRKLGFS NSNPAQDLER VPPGLLALDN MLYFSRNAPS AYSRFVLENS SREDKHECPF ARGSIQLTVL LCELLRVGEP CSETAQDFSP MFFGQDQSFH ELFCVGIQLL NKTWKEMRAT QEDFDKVMQV VREQLARTLA LKPTSLELFR TKVNALTYGE VLRLRQTERL HQEGTLAPPI LELREKLKPE LMGLIRQQRL LRLCEGTLFR KISSRRRQDK LWFCCLSPNH KLLQYGDMEE GASPPTLESL PEQLPVADMR ALLTGKDCPH VREKGSGKQN KDLYELAFSI SYDRGEEEAY LNFIAPSKRE FYLWTDGLSA LLGSPMGSEQ TRLDLEQLLT METKLRLLEL ENVPIPERPP PVPPPPTNFN FCYDCSIAEP |
| 预测分子量 | 81,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ELMO3重组蛋白相关的文献概览：
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1. **文献名称**: *Structural insights into ELMO3-mediated Rac1 activation in cancer cell invasion*
**作者**: Smith J, et al.
**摘要**: 本研究解析了ELMO3重组蛋白与Rac1 GTP酶的相互作用结构，发现ELMO3通过C端结构域招募Rac1并促进其激活，进而驱动乳腺癌细胞的迁移和侵袭。实验利用大肠杆菌表达的重组ELMO3蛋白验证了其促癌功能。
2. **文献名称**: *ELMO3 expression correlates with poor prognosis in colorectal cancer via EMT modulation*
**作者**: Chen L, et al.
**摘要**: 通过构建ELMO3重组蛋白过表达的结直肠癌细胞模型，作者证明ELMO3通过激活Wnt/β-catenin信号通路诱导上皮-间质转化(EMT)，促进肿瘤转移。重组蛋白的体外实验进一步证实其与β-catenin的物理结合。
3. **文献名称**: *Recombinant ELMO3 purification and its role in phagocytosis of apoptotic cells*
**作者**: Patel R, et al.
**摘要**: 该研究优化了ELMO3重组蛋白在昆虫细胞中的表达和纯化流程，并发现ELMO3通过与Dock180蛋白形成复合物，调控巨噬细胞对凋亡细胞的吞噬功能，揭示了其在免疫清除中的潜在作用。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“ELMO3 recombinant protein”为关键词检索最新论文。若需具体文献，可提供更详细的研究背景进一步筛选。
ELMO3 (Engulfment and Cell Motility Protein 3) is a member of the ELMO protein family, evolutionarily conserved cytosolic factors that play critical roles in regulating cellular processes such as cell migration, phagocytosis, and cytoskeletal reorganization. The ELMO family (ELMO1. ELMO2. and ELMO3) interacts with Dock-family guanine nucleotide exchange factors (GEFs) to activate Rho GTPases, particularly Rac1. which governs actin dynamics and cell motility. ELMO3. the least characterized member, shares structural homology with ELMO1/2. including a PH (Pleckstrin Homology) domain and an ELMO-specific helical domain, facilitating protein-protein interactions and membrane localization.
Recombinant ELMO3 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The recombinant form allows researchers to study ELMO3's biochemical properties, interaction partners, and signaling mechanisms in vitro. Its production often involves affinity tags (e.g., His-tag) for purification and detection.
Functionally, ELMO3 is implicated in cancer progression, inflammation, and developmental processes by modulating Rac1-dependent pathways. Overexpression of ELMO3 correlates with enhanced tumor cell invasion and metastasis in cancers like breast and colorectal cancer. Recombinant ELMO3 enables exploration of its role in disease models, drug screening, and structural studies to identify binding interfaces for therapeutic targeting. Despite its shared functions with ELMO1/2. emerging evidence suggests ELMO3 may exhibit unique spatiotemporal regulation or tissue-specific roles, warranting further investigation. As a research tool, recombinant ELMO3 contributes to unraveling the complexity of Rho GTPase signaling networks and their implications in human diseases.
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