| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY6 |
| Uniprot No | O43306 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1168aa |
| 氨基酸序列 | MSWFSGLLVPKVDERKTAWGERNGQKRSRRRGTRAGGFCTPRYMSCLRDAEPPSPTPAGPPRCPWQDDAFIRRGGPGKGKELGLRAVALGFEDTEVTTTAGGTAEVAPDAVPRSGRSCWRRLVQVFQSKQFRSAKLERLYQRYFFQMNQSSLTLLMAVLVLLTAVLLAFHAAPARPQPAYVALLACAAALFVGLMVVCNRHSFRQDSMWVVSYVVLGILAAVQVGGALAADPRSPSAGLWCPVFFVYIAYTLLPIRMRAAVLSGLGLSTLHLILAWQLNRGDAFLWKQLGANVLLFLCTNVIGICTHYPAEVSQRQAFQETRGYIQARLHLQHENRQQERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNAYLKEQHIETFLILGASQKRKEEKAMLAKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFRQMGIDDSSKDNRGTQDALNPEDEVDEFLSRAIDARSIDQLRKDHVRRFLLTFQREDLEKKYSRKVDPRFGAYVACALLVFCFICFIQLLIFPHSTLMLGIYASIFLLLLITVLICAVYSCGSLFPKALQRLSRSIVRSRAHSTAVGIFSVLLVFTSAIANMFTCNHTPIRSCAARMLNLTPADITACHLQQLNYSLGLDAPLCEGTMPTCSFPEYFIGNMLLSLLASSVFLHISSIGKLAMIFVLGLIYLVLLLLGPPATIFDNYDLLLGVHGLASSNETFDGLDCPAAGRVALKYMTPVILLVFALALYLHAQQVESTARLDFLWKLQATGEKEEMEELQAYNRRLLHNILPKDVAAHFLARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEERFRQLEKIKTIGSTYMAASGLNASTYDQVGRSHITALADYAMRLMEQMKHINEHSFNNFQMKIGLNMGPVVAGVIGARKPQYDIWGNTVNVSSRMDSTGVPDRIQVTTDLYQVLAAKGYQLECRGVVKVKGKGEMTTYFLNGGPSS |
| 预测分子量 | 130,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY6重组蛋白的3篇参考文献及其摘要概括：
1. **"Functional characterization of recombinant human ADCY6 in cAMP signaling"**
*Smith A, et al.*
该研究通过表达并纯化人源ADCY6重组蛋白，揭示了其在cAMP信号通路中的催化活性，发现其受G蛋白偶联受体(GPCR)调控，并分析了特定突变对酶活性的影响。
2. **"Structural insights into ADCY6 activation by Gαs protein"**
*Chen L, et al.*
作者利用重组ADCY6蛋白进行冷冻电镜结构解析，阐明了Gαs亚基与ADCY6的结合机制，揭示了其激活过程中构象变化的分子基础，为靶向药物设计提供了依据。
3. **"ADCY6 recombinant protein as a therapeutic target in metabolic disorders"**
*Wang Y, et al.*
研究通过体外实验证明，重组ADCY6蛋白的异常活性与肥胖相关代谢紊乱密切相关，并筛选出小分子抑制剂，提示其作为代谢疾病治疗靶点的潜力。
(注：上述文献为示例，实际引用时需核实真实论文信息。)
**Background of ADCY6 Recombinant Protein**
Adenylyl cyclase 6 (ADCY6) is a membrane-bound enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. ADCY6 is one of nine transmembrane adenylyl cyclase isoforms (ADCY1–9) in mammals and is widely expressed in tissues such as the heart, brain, and smooth muscle. It plays a pivotal role in G protein-coupled receptor (GPCR) signaling pathways, particularly those mediated by β-adrenergic receptors, regulating processes like cardiac contractility, neurotransmission, and hormone secretion.
Recombinant ADCY6 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional activity. The purified protein typically retains key domains, including the cytoplasmic C1 and C2 catalytic regions and transmembrane helices, enabling in vitro studies of its enzymatic kinetics, regulation by stimulatory (Gαs) or inhibitory (Gαi) G-proteins, and interaction with calcium/calmodulin.
Research applications of ADCY6 recombinant protein include elucidating its role in diseases linked to cAMP dysregulation, such as heart failure, hypertension, and neurological disorders. It is also used to screen small-molecule modulators for therapeutic development. Additionally, structural studies using recombinant ADCY6 help identify mechanisms of enzyme activation/inhibition and guide drug design.
Quality control involves assays verifying enzymatic activity (e.g., cAMP production via ELISA), purity (SDS-PAGE), and specificity (Western blot). By providing a standardized, biologically active form of ADCY6. recombinant proteins facilitate reproducible research into cAMP-mediated signaling and its therapeutic targeting.
In summary, ADCY6 recombinant protein is a vital tool for dissecting the molecular basis of cellular signaling and advancing drug discovery for cAMP-related pathologies.
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