| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY4 |
| Uniprot No | Q8NFM4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1077aa |
| 氨基酸序列 | MARLFSPRPPPSEDLFYETYYSLSQQYPLLLLLLGIVLCALAALLAVAWASGRELTSDPSFLTTVLCALGGFSLLLGLASREQRLQRWTRPLSGLVWVALLALGHAFLFTGGVVSAWDQVSYFLFVIFTAYAMLPLGMRDAAVAGLASSLSHLLVLGLYLGPQPDSRPALLPQLAANAVLFLCGNVAGVYHKALMERALRATFREALSSLHSRRRLDTEKKHQEHLLLSILPAYLAREMKAEIMARLQAGQGSRPESTNNFHSLYVKRHQGVSVLYADIVGFTRLASECSPKELVLMLNELFGKFDQIAKEHECMRIKILGDCYYCVSGLPLSLPDHAINCVRMGLDMCRAIRKLRAATGVDINMRVGVHSGSVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHITGATLALLAGAYAVEDAGMEHRDPYLRELGEPTYLVIDPRAEEEDEKGTAGGLLSSLEGLKMRPSLLMTRYLESWGAAKPFAHLSHGDSPVSTSTPLPEKTLASFSTQWSLDRSRTPRGLDDELDTGDAKFFQVIEQLNSQKQWKQSKDFNPLTLYFREKEMEKEYRLSAIPAFKYYEACTFLVFLSNFIIQMLVTNRPPALAITYSITFLLFLLILFVCFSEDLMRCVLKGPKMLHWLPALSGLVATRPGLRIALGTATILLVFAMAITSLFFFPTSSDCPFQAPNVSSMISNLSWELPGSLPLISVPYSMHCCTLGFLSCSLFLHMSFELKLLLLLLWLAASCSLFLHSHAWLSECLIVRLYLGPLDSRPGVLKEPKLMGAISFFIFFFTLLVLARQNEYYCRLDFLWKKKLRQEREETETMENLTRLLLENVLPAHVAPQFIGQNRRNEDLYHQSYECVCVLFASVPDFKEFYSESNINHEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNATSGQDAQQDAERSCSHLGTMVEFAVALGSKLDVINKHSFNNFRLRVGLNHGPVVAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETAWALQSLGYTCYSRGVIKVKGKGQLCTYFLNTDLTRTGPPSATLG |
| 预测分子量 | 119,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY4重组蛋白的示例参考文献(注：以下内容为虚构示例，建议通过PubMed、Google Scholar等平台检索真实文献)：
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1. **标题**: *"Functional Characterization of Recombinant ADCY4 in cAMP Signaling Pathways"*
**作者**: Smith J, et al.
**摘要**: 本研究通过在大肠杆菌系统中表达并纯化ADCY4重组蛋白，分析了其酶活性及钙离子/钙调蛋白的调控作用，揭示了ADCY4在心血管细胞cAMP信号通路中的特异性功能。
2. **标题**: *"Structural Insights into ADCY4 Catalytic Domain via Recombinant Protein Crystallography"*
**作者**: Lee H, et al.
**摘要**: 利用昆虫细胞表达系统获得高纯度ADCY4重组蛋白，通过X射线晶体学解析其催化结构域的三维结构，为靶向ADCY4的药物设计提供了结构基础。
3. **标题**: *"ADCY4 Recombinant Protein Restores Dysregulated cAMP Production in Metabolic Syndrome Models"*
**作者**: Gonzalez R, et al.
**摘要**: 在体外实验中，外源性ADCY4重组蛋白成功恢复了代谢综合征细胞模型中受损的cAMP生成能力，表明其在代谢疾病治疗中的潜在应用价值。
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**建议**：如需真实文献，可访问PubMed(https://pubmed.ncbi.nlm.nih.gov/)并搜索关键词“ADCY4 recombinant protein”或结合具体研究领域筛选。
Adenylyl cyclase type 4 (ADCY4) is a membrane-associated enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of ATP to cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. As one of nine transmembrane adenylate cyclase isoforms in mammals, ADCY4 is primarily expressed in various tissues, including the brain, heart, and smooth muscle. It plays a key role in G protein-coupled receptor (GPCR) signaling pathways, translating extracellular signals into intracellular cAMP-mediated responses that regulate processes like metabolism, gene expression, and cell proliferation.
Recombinant ADCY4 protein is engineered through genetic engineering techniques, typically expressed in mammalian or insect cell systems to preserve its post-translational modifications and functional conformation. The protein consists of two cytoplasmic catalytic domains and twelve transmembrane helices, with regulatory regions responsive to Gαs and Gαi proteins, calcium ions, and other modulators. Researchers use recombinant ADCY4 to study its activation/inhibition mechanisms, interactions with signaling molecules, and dysregulation in diseases such as heart failure, neurological disorders, and cancer. Mutations in ADCY4 have been linked to developmental defects and metabolic syndromes, making it a potential therapeutic target.
Purified recombinant ADCY4 is often tagged (e.g., His-tag, FLAG) for ease of isolation and detection. Studies leveraging this protein have advanced drug discovery efforts, particularly in designing modulators to fine-tune cAMP signaling. However, its hydrophobic transmembrane structure poses challenges in solubilization and stabilization during in vitro experiments, requiring optimized buffers and detergents. Current research continues to explore its tissue-specific roles and crosstalk with other signaling pathways, emphasizing its importance in cellular homeostasis and disease pathology.
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