| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY3 |
| Uniprot No | O60266 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 854-1144aa |
| 氨基酸序列 | YYFSRHVEKLARTLFLWKIEVHDQKERVYEMRRWNEALVTNMLPEHVARHFLGSKKRDEELYSQTYDEIGVMFASLPNFADFYTEESINNGGIECLRFLNEIISDFDSLLDNPKFRVITKIKTIGSTYMAASGVTPDVNTNGFASSNKEDKSERERWQHLADLADFALAMKDTLTNINNQSFNNFMLRIGMNKGGVLAGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEETQVILREYGFRFVRRGPIFVKGKGELLTFFLKGRDKLATFPNGPSVTLPHQVVDNS |
| 预测分子量 | 40.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY3重组蛋白的3篇示例参考文献(注：文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Functional characterization of recombinant ADCY3 in cAMP signaling pathways"
**作者**: Smith J, et al.
**摘要**: 本研究通过昆虫细胞表达系统制备重组ADCY3蛋白，验证其催化ATP生成cAMP的酶活性，并发现其活性受G蛋白偶联受体(GPCR)调控，提示ADCY3在细胞信号转导中的关键作用。
2. **文献名称**: "ADCY3 mutations disrupt lipid metabolism via impaired adipocyte differentiation"
**作者**: Chen L, et al.
**摘要**: 利用重组ADCY3蛋白及基因编辑技术，揭示ADCY3突变导致脂肪细胞分化异常，降低cAMP水平，进而影响脂质代谢通路，为肥胖相关疾病的分子机制提供新证据。
3. **文献名称**: "Structural insights into ADCY3 activation by Gαs protein"
**作者**: Kumar R, et al.
**摘要**: 通过冷冻电镜解析重组ADCY3与Gαs蛋白复合物的三维结构，阐明两者相互作用的关键结构域，为靶向ADCY3的药物设计奠定基础。
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建议通过PubMed、Google Scholar等平台检索真实文献，关键词："ADCY3 recombinant protein" 或 "adenylyl cyclase 3 expression"。
Adenylyl cyclase 3 (ADCY3) is a membrane-bound enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. ADCY3 is primarily associated with G protein-coupled receptor (GPCR) pathways, where it transduces extracellular signals into intracellular cAMP-mediated responses. This enzyme is highly expressed in olfactory cilia, brain regions, and adipose tissues, implicating its roles in odorant detection, neuronal signaling, and metabolic regulation. Dysregulation of ADCY3 has been linked to obesity, insulin resistance, and neurological disorders, making it a target for therapeutic research.
Recombinant ADCY3 protein is engineered in vitro using expression systems like mammalian cells or *E. coli* to produce a purified, functional form of the enzyme. It typically retains key structural domains, including the catalytic core and transmembrane regions, ensuring enzymatic activity. Researchers utilize recombinant ADCY3 to study its activation mechanisms, interactions with GPCRs or G proteins, and responses to inhibitors/activators. Its applications extend to drug screening, structural biology (e.g., crystallography), and disease modeling. For instance, studies using recombinant ADCY3 have elucidated its role in leptin signaling and energy homeostasis, offering insights into obesity pathways. The protein’s availability also aids in developing targeted therapies for metabolic and neurodegenerative conditions. Overall, recombinant ADCY3 serves as a vital tool for unraveling cAMP-dependent signaling networks and advancing precision medicine.
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