| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY10 |
| Uniprot No | Q96PN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY10重组蛋白的参考文献及摘要概括：
1. **"Recombinant soluble adenylyl cyclase (sAC) reveals bicarbonate-dependent signaling in somatic cells"**
- 作者：J. Buck, M.L. Levin, et al.
- 摘要：该研究通过在大肠杆菌中表达重组ADCY10(sAC)，揭示了其在体细胞中依赖碳酸氢盐(HCO₃⁻)的cAMP信号通路，阐明了sAC在代谢和细胞应激响应中的作用机制。
2. **"The catalytic mechanism of mammalian adenylyl cyclase: A structural perspective"**
- 作者：C. Ramos-Espiritu, L.R. Levin, et al.
- 摘要：通过重组ADCY10蛋白的晶体结构分析，探讨其催化活性位点及调控机制，提出HCO₃⁻和ATP结合对酶活性的协同调控模型。
3. **"Soluble adenylyl cyclase governs mitochondrial respiration and metabolic reprogramming in cancer cells"**
- 作者：L.R. Levin, J. Buck, et al.
- 摘要：利用重组ADCY10蛋白研究其在肿瘤细胞线粒体中的功能，发现sAC通过调节cAMP水平影响氧化磷酸化和糖酵解代谢重编程，为癌症治疗提供新靶点。
4. **"Bicarbonate-sensing soluble adenylyl cyclase is a pH- and redox-regulated ATPase"**
- 作者：S. Tresguerres, M. Levin, et al.
- 摘要：通过重组ADCY10蛋白的功能实验，证明其不仅是HCO₃⁻依赖性环化酶，还具有pH和氧化还原状态依赖的ATP酶活性，扩展了对其多功能的认知。
这些文献聚焦于ADCY10重组蛋白的表达、结构解析及其在代谢、信号转导和疾病中的功能机制。
ADCY10. also known as soluble adenylyl cyclase (sAC), is a unique member of the adenylate cyclase family that catalyzes the conversion of ATP to cyclic AMP (cAMP), a critical secondary messenger regulating diverse cellular processes. Unlike transmembrane adenylyl cyclases (tmACs), which are activated by G-protein-coupled receptors, ADCY10 operates independently of extracellular signals and is primarily regulated by bicarbonate (HCO₃⁻) and calcium ions (Ca²⁺). This enzyme exists in two isoforms: a full-length variant localized in mitochondria, nuclei, and cytoplasm, and a truncated soluble form. Its bicarbonate sensitivity links ADCY10 to pH and CO₂ homeostasis, making it pivotal in physiological processes such as sperm motility, neuronal signaling, and mitochondrial energy metabolism.
Recombinant ADCY10 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to study its structure, regulation, and interactions. Purification often involves affinity tags (e.g., His-tag) followed by biochemical assays to validate catalytic activity. Researchers leverage recombinant ADCY10 to explore its unique activation mechanisms, substrate specificity, and role in diseases like hormonal resistance syndromes, metabolic disorders, and cancer. Recent studies highlight its potential as a therapeutic target, particularly in conditions involving cAMP dysregulation. However, challenges remain in understanding its tissue-specific functions and crosstalk with tmAC pathways. Advances in structural biology and gene-editing tools continue to unravel ADCY10's complex roles in cellular signaling networks.
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