| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY1 |
| Uniprot No | Q08828 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1119aa |
| 氨基酸序列 | MAGAPRGGGGGGGGAGEPGGAERAAGTSRRRGLRACDEEFACPELEALFRGYTLRLEQAATLKALAVLSLLAGALALAELLGAPGPAPGLAKGSHPVHCVLFLALLVVTNVRSLQVPQLQQVGQLALLFSLTFALLCCPFALGGPARGSAGAAGGPATAEQGVWQLLLVTFVSYALLPVRSLLAIGFGLVVAASHLLVTATLVPAKRPRLWRTLGANALLFVGVNMYGVFVRILTERSQRKAFLQARSCIEDRLRLEDENEKQERLLMSLLPRNVAMEMKEDFLKPPERIFHKIYIQRHDNVSILFADIVGFTGLASQCTAQELVKLLNELFGKFDELATENHCRRIKILGDCYYCVSGLTQPKTDHAHCCVEMGLDMIDTITSVAEATEVDLNMRVGLHTGRVLCGVLGLRKWQYDVWSNDVTLANVMEAAGLPGKVHITKTTLACLNGDYEVEPGYGHERNSFLKTHNIETFFIVPSHRRKIFPGLILSDIKPAKRMKFKTVCYLLVQLMHCRKMFKAEIPFSNVMTCEDDDKRRALRTASEKLRNRSSFSTNVVYTTPGTRVNRYISRLLEARQTELEMADLNFFTLKYKHVEREQKYHQLQDEYFTSAVVLTLILAALFGLVYLLIFPQSVVVLLLLVFCICFLVACVLYLHITRVQCFPGCLTIQIRTVLCIFIVVLIYSVAQGCVVGCLPWAWSSKPNSSLVVLSSGGQRTALPTLPCESTHHALLCCLVGTLPLAIFFRVSSLPKMILLSGLTTSYILVLELSGYTRTGGGAVSGRSYEPIVAILLFSCALALHARQVDIRLRLDYLWAAQAEEEREDMEKVKLDNRRILFNLLPAHVAQHFLMSNPRNMDLYYQSYSQVGVMFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDELMEKDFYKDIEKIKTIGSTYMAAVGLAPTSGTKAKKSISSHLSTLADFAIEMFDVLDEINYQSYNDFVLRVGINVGPVVAGVIGARRPQYDIWGNTVNVASRMDSTGVQGRIQVTEEVHRLLRRCPYHFVCRGKVSVKGKGEMLTYFLEGRTDGNGSQIRSLGLDRKMCPFGRAGLQGRRPPVCPMPGVSVRAGLPPHSPGQYLPSAAAGKEA |
| 预测分子量 | 123,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY1重组蛋白的3篇示例参考文献(注：部分文献信息为示例性概括，建议通过学术数据库查询真实文献)：
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1. **文献名称**: "Recombinant expression and functional characterization of human ADCY1 in HEK293 cells"
**作者**: Zhang L, et al.
**摘要**: 本研究利用HEK293细胞系统成功表达了重组人源ADCY1蛋白，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，该重组蛋白在钙离子/钙调蛋白激活下显著提高cAMP合成活性，揭示了ADCY1在神经元信号传导中的调控机制。
2. **文献名称**: "Structural insights into ADCY1 activation by Gαs using cryo-EM"
**作者**: Chen R, et al.
**摘要**: 通过冷冻电镜技术解析了重组ADCY1与Gαs蛋白复合物的三维结构，阐明了Gαs结合诱导ADCY1催化结构域构象变化的分子机制，为靶向ADCY1的药物设计提供了结构基础。
3. **文献名称**: "Baculovirus-mediated ADCY1 production and its application in high-throughput screening"
**作者**: Müller S, et al.
**摘要**: 采用杆状病毒-昆虫细胞系统高效表达ADCY1重组蛋白，并开发了基于该蛋白的体外酶活检测体系，成功用于筛选调控cAMP通路的小分子抑制剂，验证了其在药物发现中的实用性。
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**提示**：实际研究中建议通过PubMed、Google Scholar等平台检索关键词“ADCY1 recombinant protein”或结合具体研究领域(如结构、疾病模型等)筛选文献。
**Background of ADCY1 Recombinant Protein**
Adenylate cyclase 1 (ADCY1), also known as adenylate cyclase type 1. is a membrane-bound enzyme that catalyzes the conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. It belongs to the adenylate cyclase family, which comprises multiple isoforms regulated by diverse stimuli, including G-protein-coupled receptors (GPCRs) and calcium/calmodulin signaling. ADCY1 is predominantly expressed in the brain, particularly in regions associated with learning and memory, such as the hippocampus, as well as in the heart and other tissues.
Recombinant ADCY1 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the enzyme for research applications. This protein retains key structural features, including 12 transmembrane domains and catalytic regions, enabling studies on its regulation, interaction with signaling molecules (e.g., Gαs, calmodulin), and role in cAMP-dependent pathways.
ADCY1 is implicated in synaptic plasticity, neurotransmitter release, and memory formation. Dysregulation of ADCY1 activity has been linked to neurological disorders, such as Alzheimer’s disease, and cardiac conditions. Its recombinant form facilitates *in vitro* studies to explore these mechanisms, screen therapeutic compounds, or model diseases. Additionally, ADCY1’s calcium/calmodulin sensitivity makes it a focus for understanding cross-talk between cAMP and calcium signaling.
The production of recombinant ADCY1 often includes tags (e.g., His-tag) for purification and detection, ensuring high specificity and activity for experimental reproducibility. Its application spans biochemistry, pharmacology, and neuroscience, offering insights into cellular signaling networks and potential drug targets.
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