| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY2 |
| Uniprot No | Q08462 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1091aa |
| 氨基酸序列 | MWQEAMRRRRYLRDRSEEAAGGGDGLPRSRDWLYESYYCMSQQHPLIVFLLLIVMGSCLALLAVFFALGLEVEDHVAFLITVPTALAIFFAIFILVCIESVFKKLLRLFSLVIWICLVAMGYLFMCFGGTVSPWDQVSFFLFIIFVVYTMLPFNMRDAIIASVLTSSSHTIVLSVCLSATPGGKEHLVWQILANVIIFICGNLAGAYHKHLMELALQQTYQDTCNCIKSRIKLEFEKRQQERLLLSLLPAHIAMEMKAEIIQRLQGPKAGQMENTNNFHNLYVKRHTNVSILYADIVGFTRLASDCSPGELVHMLNELFGKFDQIAKENECMRIKILGDCYYCVSGLPISLPNHAKNCVKMGLDMCEAIKKVRDATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEEGDGDIRDPYLKQHLVKTYFVINPKGERRSPQHLFRPRHTLDGAKMRASVRMTRYLESWGAAKPFAHLHHRDSMTTENGKISTTDVPMGQHNFQNRTLRTKSQKKRFEEELNERMIQAIDGINAQKQWLKSEDIQRISLLFYNKVLEKEYRATALPAFKYYVTCACLIFFCIFIVQILVLPKTSVLGISFGAAFLLLAFILFVCFAGQLLQCSKKASPLLMWLLKSSGIIANRPWPRISLTIITTAIILMMAVFNMFFLSDSEETIPPTANTTNTSFSASNNQVAILRAQNLFFLPYFIYSCILGLISCSVFLRVNYELKMLIMMVALVGYNTILLHTHAHVLGDYSQVLFERPGIWKDLKTMGSVSLSIFFITLLVLGRQNEYYCRLDFLWKNKFKKEREEIETMENLNRVLLENVLPAHVAEHFLARSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAVPSQEHSQEPERQYMHIGTMVEFAFALVGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLVLQTLGYTCTCRGIINVKGKGDLKTYFVNTEMSRSLSQSNVAS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY2重组蛋白的3篇参考文献的简要总结(注：内容为模拟生成，非真实文献)：
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1. **文献名称**: *Expression and Purification of Recombinant ADCY2 in Insect Cells for Functional Studies*
**作者**: Li, X.; Wang, H. et al.
**摘要**: 本研究描述了在杆状病毒-昆虫细胞系统中高效表达人源ADCY2重组蛋白的优化方法，并通过亲和层析和凝胶过滤技术获得高纯度蛋白。研究证实重组ADCY2具有催化ATP生成cAMP的活性，为后续酶动力学及药物筛选提供了可靠材料。
2. **文献名称**: *Cryo-EM Structure of ADCY2 Reveals G Protein Interaction Mechanisms*
**作者**: Chen, J.; Smith, R.K. et al.
**摘要**: 利用冷冻电镜技术解析了ADCY2重组蛋白与Gsα亚基复合物的三维结构(分辨率3.2 Å)，揭示了ADCY2被G蛋白偶联受体激活的构象变化机制，为靶向ADCY2的调节剂设计提供了结构基础。
3. **文献名称**: *Role of ADCY2 in Neuronal cAMP Signaling: Insights from Recombinant Protein Assays*
**作者**: Müller, T.; Schmidt, A. et al.
**摘要**: 通过体外重组ADCY2蛋白实验，研究发现其活性受钙离子和钙调蛋白的协同调控，提示ADCY2可能在海马体神经元中通过cAMP-PKA通路参与突触可塑性和记忆形成。
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**注**：如需真实文献，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索关键词“ADCY2 recombinant protein”获取最新研究。
**Background of ADCY2 Recombinant Protein**
Adenylyl cyclase 2 (ADCY2), a member of the membrane-bound adenylyl cyclase family, catalyzes the conversion of ATP to cyclic AMP (cAMP), a critical secondary messenger in cellular signaling. It is encoded by the *ADCY2* gene and plays a regulatory role in G protein-coupled receptor (GPCR)-mediated pathways, influencing processes like neurotransmission, hormone response, and cell differentiation. ADCY2 is predominantly expressed in the brain, particularly in regions associated with cognition and mood regulation, implicating its potential involvement in neurological disorders such as schizophrenia and bipolar disorder.
Recombinant ADCY2 protein is engineered through molecular cloning, typically expressed in mammalian systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional activity. This purified protein retains the catalytic domains required for cAMP synthesis, including the N-terminal transmembrane regions and the cytoplasmic C-terminal catalytic core. Researchers utilize ADCY2 recombinant protein to study its enzymatic kinetics, interactions with stimulatory/inhibitory G proteins (e.g., Gαs, Gαi), and responses to regulators like forskolin or calcium/calmodulin.
Its applications extend to drug discovery, particularly for targeting cAMP-related pathologies. Dysregulation of ADCY2 has been linked to cancers, neuropsychiatric diseases, and metabolic disorders, making it a potential therapeutic biomarker. Structural studies using recombinant ADCY2 also aid in designing modulators to fine-tune cAMP signaling. However, challenges remain in understanding isoform-specific functions due to structural similarities among adenylyl cyclases. Continued research on ADCY2 recombinant protein is vital for unraveling its physiological and pathological roles, paving the way for novel treatment strategies.
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