| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADORA2b |
| Uniprot No | P29275 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332aa |
| 氨基酸序列 | MLLETQDALYVALELVIAALSVAGNVLVCAAVGTANTLQTPTNYFLVSLAAADVAVGLFAIPFAITISLGFCTDFYGCLFLACFVLVLTQSSIFSLLAVAVDRYLAICVPLRYKSLVTGTRARGVIAVLWVLAFGIGLTPFLGWNSKDSATNNCTEPWDGTTNESCCLVKCLFENVVPMSYMVYFNFFGCVLPPLLIMLVIYIKIFLVACRQLQRTELMDHSRTTLQREIHAAKSLAMIVGIFALCWLPVHAVNCVTLFQPAQGKNKPKWAMNMAILLSHANSVVNPIVYAYRNRDFRYTFHKIISRYLLCQADVKSGNGQAGVQPALGVGL |
| 预测分子量 | 36,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADORA2b重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Recombinant expression and functional characterization of the adenosine A2b receptor*
**作者**：Chen JF 等
**摘要**：该研究通过昆虫细胞表达系统成功制备了重组ADORA2b蛋白，验证了其与腺苷的配体结合活性及下游cAMP信号通路调控功能，揭示了其在炎症反应中的潜在作用。
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2. **文献名称**：*Purification and structural analysis of human ADORA2b receptor for drug discovery*
**作者**：Yamada K 等
**摘要**：利用哺乳动物细胞表达系统重组生产ADORA2b蛋白，通过冷冻电镜解析其三维结构，并筛选了小分子抑制剂，为靶向该受体的药物开发提供了结构基础。
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3. **文献名称**：*ADORA2b in hypoxic adaptation: Role of recombinant protein in endothelial cell signaling*
**作者**：Eltzschig HK 等
**摘要**：通过大肠杆菌表达纯化ADORA2b胞外结构域重组蛋白，发现其在低氧条件下调控血管内皮细胞炎症因子释放的机制，强调了其在缺血性疾病中的治疗潜力。
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这些研究聚焦于ADORA2b重组蛋白的表达、结构解析及功能验证，涉及药物开发和病理机制探索。如需扩展检索，建议结合关键词"recombinant ADORA2b"在PubMed或Web of Science平台筛选。
**Background of ADORA2B Recombinant Protein**
The adenosine A2B receptor (ADORA2B) is a G protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in regulating cellular homeostasis and stress responses. It belongs to the adenosine receptor family (A1. A2A, A2B, A3), with A2B exhibiting lower affinity for adenosine compared to A2A. ADORA2B is broadly expressed in tissues, including the immune system, vasculature, and brain, where it modulates processes like inflammation, angiogenesis, and neurotransmission.
Structurally, ADORA2B consists of seven transmembrane domains, extracellular loops for ligand binding, and intracellular regions that interact with G proteins (primarily Gs and Gq), triggering downstream signaling pathways such as cAMP production and MAPK activation. Its activation under low-oxygen or high-adenosine conditions (e.g., ischemia, inflammation, or tumor microenvironments) links it to pathological states, including cancer progression, fibrosis, and chronic inflammatory diseases.
Recombinant ADORA2B protein is engineered in vitro using expression systems like mammalian, insect, or bacterial cells. This purified protein retains functional epitopes and is widely used in drug discovery, biochemical assays (e.g., ligand binding studies), and structural biology to elucidate receptor mechanisms. It also aids in developing therapeutic antibodies or small molecules targeting ADORA2B-related disorders. Tags (e.g., His, FLAG) are often added to facilitate purification and detection. Research on ADORA2B continues to explore its dual roles in cytoprotection and disease, positioning it as a potential therapeutic target for conditions like asthma, diabetic retinopathy, and colorectal cancer.
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