| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADORA1 |
| Uniprot No | P30542 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MPPSISAFQAAYIGIEVLIALVSVPGNVLVIWAVKVNQALRDATFCFIVSLAVADVAVGALVIPLAILINIGPQTYFHTCLMVACPVLILTQSSILALLAIAVDRYLRVKIPLRYKMVVTPRRAAVAIAGCWILSFVVGLTPMFGWNNLSAVERAWAANGSMGEPVIKCEFEKVISMEYMVYFNFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSASSGDPQKYYGKELKIAKSLALILFLFALSWLPLHILNCITLFCPSCHKPSILTYIAIFLTHGNSAMNPIVYAFRIQKFRVTFLKIWNDHFRCQPAPPIDEDLPEERPDD |
| 预测分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADORA1重组蛋白的3篇代表性文献(基于假设性示例，实际文献需通过数据库核实)：
---
1. **文献名称**：*Cryo-EM structure of the adenosine A1 receptor coupled to an engineered heterotrimeric G protein*
**作者**：Draper-Joyce, C.J., et al.
**摘要**：通过冷冻电镜解析了ADORA1重组蛋白与工程化G蛋白复合物的三维结构，揭示了受体激活机制及腺苷结合位点的构象变化，为靶向药物设计提供结构依据。
---
2. **文献名称**：*Expression and functional analysis of recombinant human adenosine A1 receptors in insect cells*
**作者**：Klotz, K.N., et al.
**摘要**：在昆虫细胞系统中高效表达ADORA1重组蛋白，验证其与腺苷类似物的结合活性及信号转导功能，证实其在体外药物筛选中的适用性。
---
3. **文献名称**：*Stabilization of the adenosine A1 receptor by fusion proteins for biophysical studies*
**作者**：Staus, D.P., et al.
**摘要**：通过融合蛋白策略提高ADORA1重组蛋白的稳定性，成功用于核磁共振(NMR)研究，阐明其动态构象变化与配体效应的关系。
---
4. **文献名称**：*High-throughput screening of adenosine A1 receptor agonists using a recombinant luciferase reporter system*
**作者**：Gao, Z.G., et al.
**摘要**：构建基于ADORA1重组蛋白的荧光素酶报告基因系统，实现高通量筛选新型激动剂，并揭示配体选择性对受体下游信号的影响。
---
**注**：以上为模拟文献，实际引用时请通过PubMed或Web of Science检索真实文献(关键词：ADORA1. recombinant expression, structure, ligand binding)。
**Background of ADORA1 Recombinant Protein**
The adenosine A1 receptor (ADORA1) is a G protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in numerous physiological processes. It is widely expressed in the central nervous system, heart, kidneys, and adipose tissue, playing key roles in modulating neurotransmission, cardiovascular function, and metabolic regulation. ADORA1 activation typically inhibits adenylate cyclase activity via Gi/o proteins, reducing intracellular cAMP levels, and influences ion channel activity (e.g., potassium and calcium channels), contributing to its neuroprotective, anti-inflammatory, and cardioprotective effects.
Recombinant ADORA1 protein is engineered in vitro using heterologous expression systems, such as mammalian cells (HEK293 or CHO) or insect cells (baculovirus systems), to produce a purified, functional form of the receptor. These systems enable precise control over post-translational modifications (e.g., glycosylation) critical for ligand binding and signaling. The recombinant protein often includes affinity tags (e.g., His-tag, FLAG-tag) to facilitate purification and detection.
ADORA1 recombinant proteins are essential tools for structural studies (e.g., X-ray crystallography, cryo-EM), ligand-binding assays, and drug discovery. They help elucidate receptor-ligand interactions, screen potential therapeutics targeting conditions like arrhythmias, neurodegenerative diseases (e.g., Alzheimer’s), and chronic pain, and investigate signaling mechanisms. Challenges in production include maintaining receptor stability and native conformation during purification. Nonetheless, recombinant ADORA1 remains pivotal in advancing research on adenosine receptor biology and therapeutic applications.
×
