| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADORA2a |
| Uniprot No | P29274 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS |
| 预测分子量 | 44,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADORA2a重组蛋白的3篇参考文献示例(基于公开研究整理，非真实文献)：
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1. **文献名称**：Expression and Purification of Functional Human Adenosine A2a Receptor (ADORA2a) in E. coli
**作者**：Smith J. et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效表达人源ADORA2a重组蛋白的方法，利用His标签亲和层析纯化，并通过放射性配体结合实验验证其与腺苷类似物的结合活性，为后续结构研究奠定基础。
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2. **文献名称**：Structural Insights into ADORA2a Activation Mechanism via Recombinant Protein Crystallography
**作者**：Chen L. et al.
**摘要**：作者通过哺乳动物细胞表达系统获得高纯度ADORA2a重组蛋白，解析了其与激动剂复合物的晶体结构，揭示了受体激活过程中跨膜结构域的构象变化，为靶向药物设计提供结构依据。
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3. **文献名称**：ADORA2a Recombinant Protein Modulates cAMP Signaling in Parkinson’s Disease Models
**作者**：Garcia R. et al.
**摘要**：研究利用昆虫细胞表达的ADORA2a重组蛋白，证明其与多巴胺D2受体在cAMP信号通路中的交互作用，并验证其在帕金森病细胞模型中调控神经保护效应的潜在机制。
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(注：以上文献为模拟示例，实际引用时请通过学术数据库核实具体研究。)
ADORA2A, or adenosine A2a receptor, is a G protein-coupled receptor (GPCR) that plays a critical role in mediating the physiological effects of adenosine, a purine nucleoside involved in cellular energy metabolism and signaling. Primarily expressed in the brain, immune cells, and cardiovascular tissues, ADORA2A regulates diverse processes such as neurotransmitter release, immune response modulation, and vasodilation. It is particularly abundant in the striatum, where it interacts with dopamine receptors to influence motor control, cognition, and reward pathways. Dysregulation of ADORA2A signaling has been linked to neurological disorders (e.g., Parkinson’s disease, schizophrenia), inflammatory conditions, and cardiovascular diseases.
Recombinant ADORA2A protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor proteins for research and drug discovery. This recombinant form retains key structural features, including seven transmembrane domains and ligand-binding sites, enabling studies on receptor activation, ligand interactions, and downstream signaling pathways. Its production often involves tagging (e.g., His-tag) for simplified purification via affinity chromatography.
Researchers utilize recombinant ADORA2A to screen therapeutic compounds targeting adenosine receptors, such as antagonists for Parkinson’s disease or anti-inflammatory agents. It also aids in structural biology (e.g., X-ray crystallography, cryo-EM) to resolve receptor-ligand complexes, advancing the design of subtype-selective drugs. Additionally, it serves as a tool to investigate cross-talk with other receptors (e.g., dopamine D2 receptors) and adenosine’s role in tumor immunology, where ADORA2A inhibition enhances anti-cancer T-cell responses. Its recombinant availability accelerates mechanistic studies and translational applications in neurology, immunology, and cardiology.
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