| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OC |
| Uniprot No | Q16625 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522aa |
| 氨基酸序列 | MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPED EILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGG SVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFI AALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPT AQSSGSLYGSQIYALCNQFYTPAATGLYVDQYSYHYCVVDPQEAIAIVLG FMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKN VSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEV VQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYET DYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSEL DEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHC KQLKSKLSHIKKMVGDYDRQKT |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OC(骨钙素)重组蛋白的3篇示例参考文献，涵盖不同研究方向：
1. **文献名称**：*"Expression and Purification of Recombinant Osteocalcin in Escherichia coli for Bone Metabolism Studies"*
**作者**：Zhang, L., et al.
**摘要**：该研究建立了在大肠杆菌中高效表达重组OC蛋白的体系，通过优化表达条件及亲和层析纯化，获得了高纯度OC，为骨代谢机制研究提供了工具。
2. **文献名称**：*"Functional Analysis of Recombinant Osteocalcin in Osteoblast Differentiation and Bone Formation"*
**作者**：Lee, S., & Kim, H.
**摘要**：利用哺乳动物细胞表达的重组OC，作者证明其能显著促进成骨细胞分化，并通过激活Wnt/β-catenin信号通路增强骨形成活性。
3. **文献名称**：*"Application of Recombinant Osteocalcin in Biomimetic Bone Tissue Engineering Scaffolds"*
**作者**：Smith, J., et al.
**摘要**：研究将重组OC与羟基磷灰石复合材料结合，发现其显著提升支架的成骨性能，为骨缺损修复提供了新型生物材料策略。
4. **文献名称**：*"Recombinant Osteocalcin Modulates Energy Metabolism via GPRC6A Signaling in Mice"*
**作者**：Garcia, M., et al.
**摘要**：通过动物实验验证重组OC通过GPRC6A受体调控胰岛素敏感性和能量代谢，揭示了OC在内分泌调节中的新功能。
(注：上述文献为示例，实际引用需根据具体研究检索真实数据库如PubMed、Web of Science等。)
Osteocalcin (OC), a small non-collagenous protein predominantly produced by osteoblasts, plays a critical role in bone metabolism and energy homeostasis. As the most abundant non-collagenous protein in bone matrix, it constitutes ~2% of total bone protein and is widely recognized as a biomarker for bone formation. Mature OC contains three γ-carboxyglutamic acid (Gla) residues, a post-translational modification dependent on vitamin K, which enables its calcium-binding capacity for hydroxyapatite crystallization regulation. Interestingly, emerging studies reveal its endocrine functions in regulating glucose metabolism, male fertility, and cognitive processes.
Recombinant OC production addresses the limitations of natural extraction (low yield, high cost) and enables precise biomedical research. Using genetic engineering techniques, OC genes are cloned into expression vectors (commonly E. coli or mammalian systems) to produce standardized batches. Bacterial systems offer high yield but require subsequent γ-carboxylation for functional activity, while mammalian cells (e.g., HEK293) achieve proper post-translational modifications at the expense of lower productivity.
Applications span multiple fields: (1) Bone disease research: Studying osteoporosis and fracture healing mechanisms; (2) Biomaterial development: Enhancing bone graft integration; (3) Metabolic studies: Investigating its cross-talk with insulin secretion and adipose tissue; (4) Diagnostic tools: Serving as a quantifiable marker in bone turnover assays. Current challenges include optimizing cost-effective γ-carboxylation methods and elucidating receptor-mediated signaling pathways. As research progresses, recombinant OC holds promise for therapeutic interventions in metabolic disorders and age-related bone loss.
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